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dc.contributor.authorLaw, Julie A.
dc.contributor.authorAusín, Israel
dc.contributor.authorJohnson, Lianna M.
dc.contributor.authorVashisht, Ajay A Amar
dc.contributor.authorZhu, Jian-Kang
dc.contributor.authorWohlschlegel, James A A.
dc.contributor.authorJacobsen, Steven E.
dc.date.accessioned2015-08-02T09:12:20Z
dc.date.available2015-08-02T09:12:20Z
dc.date.issued2010-04-23
dc.identifier.citationLaw, J. A., Ausin, I., Johnson, L. M., Vashisht, A. A., Zhu, J.-K., Wohlschlegel, J. A., & Jacobsen, S. E. (2010). A Protein Complex Required for Polymerase V Transcripts and RNA- Directed DNA Methylation in Arabidopsis. Current Biology, 20(10), 951–956. doi:10.1016/j.cub.2010.03.062
dc.identifier.issn09609822
dc.identifier.pmid20409711
dc.identifier.doi10.1016/j.cub.2010.03.062
dc.identifier.urihttp://hdl.handle.net/10754/561476
dc.description.abstractDNA methylation is an epigenetic modification associated with gene silencing. In Arabidopsis, DNA methylation is established by DOMAINS REARRANGED METHYLTRANSFERASE 2 (DRM2), which is targeted by small interfering RNAs through a pathway termed RNA-directed DNA methylation (RdDM) [1, 2]. Recently, RdDM was shown to require intergenic noncoding (IGN) transcripts that are dependent on the Pol V polymerase. These transcripts are proposed to function as scaffolds for the recruitment of downstream RdDM proteins, including DRM2, to loci that produce both siRNAs and IGN transcripts [3]. However, the mechanism(s) through which Pol V is targeted to specific genomic loci remains largely unknown. Through affinity purification of two known RdDM components, DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 1 (DRD1) [4] and DEFECTIVE IN MERISTEM SILENCING 3 (DMS3) [5, 6], we found that they copurify with each other and with a novel protein, RNA-DIRECTED DNA METHYLATION 1 (RDM1), forming a complex we term DDR. We also found that DRD1 copurified with Pol V subunits and that RDM1, like DRD1 [3] and DMS3 [7], is required for the production of Pol V-dependent transcripts. These results suggest that the DDR complex acts in RdDM at a step upstream of the recruitment or activation of Pol V. © 2010 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipWe thank T. LaGrange for providing the NRPE1 antibody and members of the Jacobsen laboratory for helpful discussion. Jacobsen lab research was supported by U.S. National Institutes of Health (NIH) grant GM60398. I.A. was supported by a postdoctoral fellowship from the Ministerio de Educacion y Ciencia. J.A.L. was supported the NIH National Research Service Award 5F32GM820453. Wohlschlegel lab research was supported by University of California, Los Angeles Jonsson Cancer Center. S.E.J. is an investigator of the Howard Hughes Medical Institute. S.E.J., JAW., J.A.L., and I.A designed the experiments; J.A.L, I.A., L.M.J, and A.A.V. performed the experiments; J.K.Z. provided the ros1-1 rdm1-1 mutant allele and the RDM1 antibody; J.A.L. wrote the paper.
dc.publisherElsevier BV
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2972704
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2972704
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in [JournalTitle]. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in [JournalTitle], [[Volume], [Issue], (2010-04-23)] DOI: 10.1016/j.cub.2010.03.062 . © 2010. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsThis file is an open access version redistributed from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2972704
dc.subjectDNA
dc.subjectRNA
dc.titleA Protein Complex Required for Polymerase V Transcripts and RNA- Directed DNA Methylation in Arabidopsis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.identifier.journalCurrent Biology
dc.identifier.pmcidPMC2972704
dc.rights.embargodate2011-04-23
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Molecular Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, CA 90095, United States
dc.contributor.institutionLife Sciences Core Curriculum, University of California Los Angeles, Los Angeles, CA 90095, United States
dc.contributor.institutionDepartment of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, United States
dc.contributor.institutionDepartment of Biological Chemistry, David Geffen School of Medicine, UCLA, BSRB-377A, 615 Charles E. Young Dr. South, Los Angeles, CA 90095-1737, United States
dc.contributor.institutionHoward Hughes Medical Institute, University of California at Los Angeles, Los Angeles, CA 90095, United States
kaust.personZhu, Jian-Kang
refterms.dateFOA2020-04-23T12:56:06Z


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