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dc.contributor.authorRen, Zhonghai
dc.contributor.authorZheng, Zhimin
dc.contributor.authorChinnusamy, Viswanathan
dc.contributor.authorZhu, Jianhua
dc.contributor.authorCui, Xinping
dc.contributor.authorIida, Kei
dc.contributor.authorZhu, Jian-Kang
dc.date.accessioned2015-08-02T09:12:02Z
dc.date.available2015-08-02T09:12:02Z
dc.date.issued2010-03-08
dc.identifier.issn00278424
dc.identifier.pmid20212128
dc.identifier.doi10.1073/pnas.0910798107
dc.identifier.urihttp://hdl.handle.net/10754/561464
dc.description.abstractSoil salinity limits agricultural production and is a major obstacle for feeding the growing world population. We used natural genetic variation in salt tolerance among different Arabidopsis accessions to map a major quantitative trait locus (QTL) for salt tolerance and abscisic acid (ABA) sensitivity during seed germination and early seedling growth. A recombinant inbred population derived from Landsberg erecta (Ler; salt and ABA sensitive) x Shakdara (Sha; salt and ABA resistant) was used for QTL mapping. High-resolution mapping and cloning of this QTL, Response to ABA and Salt 1 (RAS1), revealed that it is an ABA- and salt stress-inducible gene and encodes a previously undescribed plant-specific protein. A premature stop codon results in a truncated RAS1 protein in Sha. Reducing the expression of RAS1 by transfer-DNA insertion in Col or RNA interference in Ler leads to decreased salt and ABA sensitivity, whereas overexpression of the Ler allele but not the Sha allele causes increased salt and ABA sensitivity. Our results suggest that RAS1 functions as a negative regulator of salt tolerance during seed germination and early seedling growth by enhancing ABA sensitivity and that its loss of function contributes to the increased salt tolerance of Sha.
dc.description.sponsorshipWe thank Maarten Koornneef for kindly providing the Ler x Sha RIL population and Hong-Xuan Lin for valuable advice on QTL analysis. This work was supported by National Institutes of Health Grants R01GM070795 and R01GM059138 and by National Science Foundation Grant IBN0420152 ( to J.-K.Z). Z.Z. was supported by the China Scholarship Council.
dc.publisherProceedings of the National Academy of Sciences
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851765
dc.subjectAbscisic acid response
dc.subjectGermination
dc.subjectNatural variation
dc.subjectQuantitative trait locus
dc.titleRAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis
dc.typeArticle
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalProceedings of the National Academy of Sciences
dc.identifier.pmcidPMC2851765
dc.contributor.institutionCenter for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, CA 92521, United States
dc.contributor.institutionKey Laboratory of Plant Pathology, Department of Plant Pathology, China Agricultural University, Beijing 100094, China
dc.contributor.institutionDepartment of Statistics, University of California, Riverside, CA 92521, United States
dc.contributor.institutionDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, United States
dc.contributor.institutionCollege of Horticulture Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, China
kaust.personZheng, Zhimin
kaust.personChinnusamy, Viswanathan
kaust.personZhu, Jian-Kang


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