RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Online Publication Date2010-03-08
Print Publication Date2010-03-23
Permanent link to this recordhttp://hdl.handle.net/10754/561464
MetadataShow full item record
AbstractSoil salinity limits agricultural production and is a major obstacle for feeding the growing world population. We used natural genetic variation in salt tolerance among different Arabidopsis accessions to map a major quantitative trait locus (QTL) for salt tolerance and abscisic acid (ABA) sensitivity during seed germination and early seedling growth. A recombinant inbred population derived from Landsberg erecta (Ler; salt and ABA sensitive) x Shakdara (Sha; salt and ABA resistant) was used for QTL mapping. High-resolution mapping and cloning of this QTL, Response to ABA and Salt 1 (RAS1), revealed that it is an ABA- and salt stress-inducible gene and encodes a previously undescribed plant-specific protein. A premature stop codon results in a truncated RAS1 protein in Sha. Reducing the expression of RAS1 by transfer-DNA insertion in Col or RNA interference in Ler leads to decreased salt and ABA sensitivity, whereas overexpression of the Ler allele but not the Sha allele causes increased salt and ABA sensitivity. Our results suggest that RAS1 functions as a negative regulator of salt tolerance during seed germination and early seedling growth by enhancing ABA sensitivity and that its loss of function contributes to the increased salt tolerance of Sha.
CitationRen, Z., Zheng, Z., Chinnusamy, V., Zhu, J., Cui, X., Iida, K., & Zhu, J.-K. (2010). RAS1, a quantitative trait locus for salt tolerance and ABA sensitivity in Arabidopsis. Proceedings of the National Academy of Sciences, 107(12), 5669–5674. doi:10.1073/pnas.0910798107
SponsorsWe thank Maarten Koornneef for kindly providing the Ler x Sha RIL population and Hong-Xuan Lin for valuable advice on QTL analysis. This work was supported by National Institutes of Health Grants R01GM070795 and R01GM059138 and by National Science Foundation Grant IBN0420152 ( to J.-K.Z). Z.Z. was supported by the China Scholarship Council.
PubMed Central IDPMC2851765
- Isolation and functional characterization of the Arabidopsis salt-tolerance 32 (AtSAT32) gene associated with salt tolerance and ABA signaling.
- Authors: Park MY, Chung MS, Koh HS, Lee DJ, Ahn SJ, Kim CS
- Issue date: 2009 Apr
- ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana.
- Authors: Sakamoto H, Matsuda O, Iba K
- Issue date: 2008 Nov
- Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana.
- Authors: Ding Z, Li S, An X, Liu X, Qin H, Wang D
- Issue date: 2009 Jan
- The Arabidopsis AP2/ERF transcription factor RAP2.6 participates in ABA, salt and osmotic stress responses.
- Authors: Zhu Q, Zhang J, Gao X, Tong J, Xiao L, Li W, Zhang H
- Issue date: 2010 Jun 1
- Nuclear-localized AtHSPR links abscisic acid-dependent salt tolerance and antioxidant defense in Arabidopsis.
- Authors: Yang T, Zhang L, Hao H, Zhang P, Zhu H, Cheng W, Wang Y, Wang X, Wang C
- Issue date: 2015 Dec