Show simple item record

dc.contributor.authorDe Jonge, Niels
dc.contributor.authorSougrat, Rachid
dc.contributor.authorNorthan, Brian M.
dc.contributor.authorPennycook, Stephen J.
dc.date.accessioned2015-08-02T09:11:50Z
dc.date.available2015-08-02T09:11:50Z
dc.date.issued2010-01-18
dc.identifier.issn14319276
dc.identifier.pmid20082729
dc.identifier.doi10.1017/S1431927609991280
dc.identifier.urihttp://hdl.handle.net/10754/561456
dc.description.abstractA three-dimensional (3D) reconstruction of the cytoskeleton and a clathrin-coated pit in mammalian cells has been achieved from a focal-series of images recorded in an aberration-corrected scanning transmission electron microscope (STEM). The specimen was a metallic replica of the biological structure comprising Pt nanoparticles 2-3 nm in diameter, with a high stability under electron beam radiation. The 3D dataset was processed by an automated deconvolution procedure. The lateral resolution was 1.1 nm, set by pixel size. Particles differing by only 10 nm in vertical position were identified as separate objects with greater than 20% dip in contrast between them. We refer to this value as the axial resolution of the deconvolution or reconstruction, the ability to recognize two objects, which were unresolved in the original dataset. The resolution of the reconstruction is comparable to that achieved by tilt-series transmission electron microscopy. However, the focal-series method does not require mechanical tilting and is therefore much faster. 3D STEM images were also recorded of the Golgi ribbon in conventional thin sections containing 3T3 cells with a comparable axial resolution in the deconvolved dataset. © 2010 Microscopy Society of America.
dc.publisherCambridge University Press (CUP)
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2917646
dc.subjectAberration-corrected STEM
dc.subjectBiological electron microscopy
dc.subjectClathrin-coated pit
dc.subjectCytoskeleton
dc.subjectDeconvolution
dc.subjectNanoparticles
dc.subjectThin sections
dc.subjectThree-dimensional electron microscopy
dc.titleThree-Dimensional scanning transmission electron microscopy of biological specimens
dc.typeArticle
dc.contributor.departmentImaging and Characterization Core Lab
dc.contributor.departmentCore Labs
dc.identifier.journalMicroscopy and Microanalysis
dc.identifier.pmcidPMC2917646
dc.contributor.institutionVanderbilt University Medical Center, Department of Molecular Physiology and Biophysics, Light Hall 702, Nashville, TN 37232-0615, United States
dc.contributor.institutionOak Ridge National Laboratory, Materials Science and Technology Division, 1 Bethel Valley Rd., Oak Ridge, TN 37831-6064, United States
dc.contributor.institutionCell Biology and Metabolism Branch, NICHD, National Institute of Health, 18 Library Drive, Bethesda, MD 20892-5430, United States
dc.contributor.institutionMedia Cybernetics Inc., 4340 East-West Hwy, Bethesda, MD 20814-4411, United States
kaust.personSougrat, Rachid
dc.date.published-online2010-01-18
dc.date.published-print2010-02


This item appears in the following Collection(s)

Show simple item record