RoboSCell: An automated single cell arraying and analysis instrument
KAUST DepartmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Electrical Engineering Program
Physical Science and Engineering (PSE) Division
Online Publication Date2009-09-09
Print Publication Date2009-12
Permanent link to this recordhttp://hdl.handle.net/10754/561431
MetadataShow full item record
AbstractSingle cell research has the potential to revolutionize experimental methods in biomedical sciences and contribute to clinical practices. Recent studies suggest analysis of single cells reveals novel features of intracellular processes, cell-to-cell interactions and cell structure. The methods of single cell analysis require mechanical resolution and accuracy that is not possible using conventional techniques. Robotic instruments and novel microdevices can achieve higher throughput and repeatability; however, the development of such instrumentation is a formidable task. A void exists in the state-of-the-art for automated analysis of single cells. With the increase in interest in single cell analyses in stem cell and cancer research the ability to facilitate higher throughput and repeatable procedures is necessary. In this paper, a high-throughput, single cell microarray-based robotic instrument, called the RoboSCell, is described. The proposed instrument employs a partially transparent single cell microarray (SCM) integrated with a robotic biomanipulator for in vitro analyses of live single cells trapped at the array sites. Cells, labeled with immunomagnetic particles, are captured at the array sites by channeling magnetic fields through encapsulated permalloy channels in the SCM. The RoboSCell is capable of systematically scanning the captured cells temporarily immobilized at the array sites and using optical methods to repeatedly measure extracellular and intracellular characteristics over time. The instrument's capabilities are demonstrated by arraying human T lymphocytes and measuring the uptake dynamics of calcein acetoxymethylester-all in a fully automated fashion. © 2009 Springer Science+Business Media, LLC.
CitationSakaki, K., Foulds, I. G., Liu, W., Dechev, N., Burke, R. D., & Park, E. J. (2009). RoboSCell: an automated single cell arraying and analysis instrument. Biomedical Microdevices, 11(6), 1317–1330. doi:10.1007/s10544-009-9351-3
- A novel permalloy based magnetic single cell micro array.
- Authors: Liu W, Dechev N, Foulds IG, Burke R, Parameswaran A, Park EJ
- Issue date: 2009 Aug 21
- Development of an autonomous biological cell manipulator with single-cell electroporation and visual servoing capabilities.
- Authors: Sakaki K, Dechev N, Burke RD, Park EJ
- Issue date: 2009 Aug
- Microfabricated platform for studying stem cell fates.
- Authors: Chin VI, Taupin P, Sanga S, Scheel J, Gage FH, Bhatia SN
- Issue date: 2004 Nov 5
- Enhancing the reliability and throughput of neurosphere culture on hydrogel microwell arrays.
- Authors: Cordey M, Limacher M, Kobel S, Taylor V, Lutolf MP
- Issue date: 2008 Oct
- An automated robotic approach with redundant navigation for minimal invasive extended transsphenoidal skull base surgery.
- Authors: Bumm K, Wurm J, Rachinger J, Dannenmann T, Bohr C, Fahlbusch R, Iro H, Nimsky C
- Issue date: 2005 Jun