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    RoboSCell: An automated single cell arraying and analysis instrument

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    Type
    Article
    Authors
    Sakaki, Kelly
    Foulds, Ian G.
    Liu, William
    Dechev, Nikolai
    Burke, Robert Douglas
    Park, Edward
    KAUST Department
    Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
    Electrical Engineering Program
    Physical Science and Engineering (PSE) Division
    Date
    2009-09-09
    Online Publication Date
    2009-09-09
    Print Publication Date
    2009-12
    Permanent link to this record
    http://hdl.handle.net/10754/561431
    
    Metadata
    Show full item record
    Abstract
    Single cell research has the potential to revolutionize experimental methods in biomedical sciences and contribute to clinical practices. Recent studies suggest analysis of single cells reveals novel features of intracellular processes, cell-to-cell interactions and cell structure. The methods of single cell analysis require mechanical resolution and accuracy that is not possible using conventional techniques. Robotic instruments and novel microdevices can achieve higher throughput and repeatability; however, the development of such instrumentation is a formidable task. A void exists in the state-of-the-art for automated analysis of single cells. With the increase in interest in single cell analyses in stem cell and cancer research the ability to facilitate higher throughput and repeatable procedures is necessary. In this paper, a high-throughput, single cell microarray-based robotic instrument, called the RoboSCell, is described. The proposed instrument employs a partially transparent single cell microarray (SCM) integrated with a robotic biomanipulator for in vitro analyses of live single cells trapped at the array sites. Cells, labeled with immunomagnetic particles, are captured at the array sites by channeling magnetic fields through encapsulated permalloy channels in the SCM. The RoboSCell is capable of systematically scanning the captured cells temporarily immobilized at the array sites and using optical methods to repeatedly measure extracellular and intracellular characteristics over time. The instrument's capabilities are demonstrated by arraying human T lymphocytes and measuring the uptake dynamics of calcein acetoxymethylester-all in a fully automated fashion. © 2009 Springer Science+Business Media, LLC.
    Citation
    Sakaki, K., Foulds, I. G., Liu, W., Dechev, N., Burke, R. D., & Park, E. J. (2009). RoboSCell: an automated single cell arraying and analysis instrument. Biomedical Microdevices, 11(6), 1317–1330. doi:10.1007/s10544-009-9351-3
    Publisher
    Springer Nature
    Journal
    Biomedical Microdevices
    DOI
    10.1007/s10544-009-9351-3
    PubMed ID
    19763834
    ae974a485f413a2113503eed53cd6c53
    10.1007/s10544-009-9351-3
    Scopus Count
    Collections
    Articles; Physical Science and Engineering (PSE) Division; Electrical and Computer Engineering Program; Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division

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