Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool
Wong, Yue Him
Bajic, Vladimir B.
Al-Suwailem, Abdulaziz M.
KAUST DepartmentApplied Mathematics and Computational Science Program
Coastal and Marine Resources Core Lab
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
KAUST Global Partnership Program
Material Science and Engineering Program
Physical Science and Engineering (PSE) Division
Online Publication Date2015-08-17
Print Publication Date2015-10
Permanent link to this recordhttp://hdl.handle.net/10754/561085
MetadataShow full item record
AbstractThe biology of biofilm in deep-sea environments is barely being explored. Here, biofilms were developed at the brine pool (characterized by limited carbon sources) and the normal bottom water adjacent to Thuwal cold seeps. Comparative metagenomics based on 50 Gb datasets identified polysaccharide degradation, nitrate reduction, and proteolysis as enriched functional categories for brine biofilms. The genomes of two dominant species: a novel deltaproteobacterium and a novel epsilonproteobacterium in the brine biofilms were reconstructed. Despite rather small genome sizes, the deltaproteobacterium possessed enhanced polysaccharide fermentation pathways, whereas the epsilonproteobacterium was a versatile nitrogen reactor possessing nar, nap and nif gene clusters. These metabolic functions, together with specific regulatory and hypersaline-tolerant genes, made the two bacteria unique compared with their close relatives including those from hydrothermal vents. Moreover, these functions were regulated by biofilm development, as both the abundance and the expression level of key functional genes were higher in later-stage biofilms, and co-occurrences between the two dominant bacteria were demonstrated. Collectively, unique mechanisms were revealed: i) polysaccharides fermentation, proteolysis interacted with nitrogen cycling to form a complex chain for energy generation; ii) remarkably, exploiting and organizing niche-specific functions would be an important strategy for biofilm-dependent adaptation to the extreme conditions.
CitationSynchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool 2015:n/a Environmental Microbiology
CollectionsArticles; Coastal and Marine Resources Core Lab; Applied Mathematics and Computational Science Program; Physical Science and Engineering (PSE) Division; Material Science and Engineering Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
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