Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool
Type
ArticleAuthors
Zhang, WeipengWang, Yong
Bougouffa, Salim

Tian, Renmao
Cao, Huiluo
Li, Yongxin
Cai, Lin
Wong, Yue Him
Zhang, Gen
Zhou, Guowei
Zhang, Xixiang

Bajic, Vladimir B.

Al-Suwailem, Abdulaziz M.
Qian, Pei-Yuan

KAUST Department
Applied Mathematics and Computational Science ProgramCoastal and Marine Resources Core Lab
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
KAUST Global Partnership Program
Material Science and Engineering Program
Physical Science and Engineering (PSE) Division
Date
2015-08-17Online Publication Date
2015-08-17Print Publication Date
2015-10Permanent link to this record
http://hdl.handle.net/10754/561085
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Show full item recordAbstract
The biology of biofilm in deep-sea environments is barely being explored. Here, biofilms were developed at the brine pool (characterized by limited carbon sources) and the normal bottom water adjacent to Thuwal cold seeps. Comparative metagenomics based on 50 Gb datasets identified polysaccharide degradation, nitrate reduction, and proteolysis as enriched functional categories for brine biofilms. The genomes of two dominant species: a novel deltaproteobacterium and a novel epsilonproteobacterium in the brine biofilms were reconstructed. Despite rather small genome sizes, the deltaproteobacterium possessed enhanced polysaccharide fermentation pathways, whereas the epsilonproteobacterium was a versatile nitrogen reactor possessing nar, nap and nif gene clusters. These metabolic functions, together with specific regulatory and hypersaline-tolerant genes, made the two bacteria unique compared with their close relatives including those from hydrothermal vents. Moreover, these functions were regulated by biofilm development, as both the abundance and the expression level of key functional genes were higher in later-stage biofilms, and co-occurrences between the two dominant bacteria were demonstrated. Collectively, unique mechanisms were revealed: i) polysaccharides fermentation, proteolysis interacted with nitrogen cycling to form a complex chain for energy generation; ii) remarkably, exploiting and organizing niche-specific functions would be an important strategy for biofilm-dependent adaptation to the extreme conditions.Citation
Synchronized dynamics of bacterial niche-specific functions during biofilm development in a cold seep brine pool 2015:n/a Environmental MicrobiologyPublisher
WileyJournal
Environmental MicrobiologyPubMed ID
26171930Additional Links
http://doi.wiley.com/10.1111/1462-2920.12978ae974a485f413a2113503eed53cd6c53
10.1111/1462-2920.12978
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Articles; Coastal and Marine Resources Core Lab; Applied Mathematics and Computational Science Program; Physical Science and Engineering (PSE) Division; Material Science and Engineering Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Science and Engineering (CEMSE) DivisionRelated articles
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