The Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stress
Bangarusamy, Dhinoth Kumar
KAUST DepartmentBioscience Core Lab
MetadataShow full item record
AbstractSalt and drought stress severely reduce plant growth and crop productivity worldwide. The identification of genes underlying stress response and tolerance is the subject of intense research in plant biology. Through microarray analyses, we previously identified in potato (Solanum tuberosum) StRGGA, coding for an Arginine Glycine Glycine (RGG) box-containing RNA-binding protein, whose expression was specifically induced in potato cell cultures gradually exposed to osmotic stress. Here, we show that the Arabidopsis (Arabidopsis thaliana) ortholog, AtRGGA, is a functional RNA-binding protein required for a proper response to osmotic stress. AtRGGA gene expression was up-regulated in seedlings after long-term exposure to abscisic acid (ABA) and polyethylene glycol, while treatments with NaCl resulted in AtRGGA down-regulation. AtRGGA promoter analysis showed activity in several tissues, including stomata, the organs controlling transpiration. Fusion of AtRGGA with yellow fluorescent protein indicated that AtRGGA is localized in the cytoplasm and the cytoplasmic perinuclear region. In addition, the rgga knockout mutant was hypersensitive to ABA in root growth and survival tests and to salt stress during germination and at the vegetative stage. AtRGGA-overexpressing plants showed higher tolerance to ABA and salt stress on plates and in soil, accumulating lower levels of proline when exposed to drought stress. Finally, a global analysis of gene expression revealed extensive alterations in the transcriptome under salt stress, including several genes such as ASCORBATE PEROXIDASE2, GLUTATHIONE S-TRANSFERASE TAU9, and several SMALL AUXIN UPREGULATED RNA-like genes showing opposite expression behavior in transgenic and knockout plants. Taken together, our results reveal an important role of AtRGGA in the mechanisms of plant response and adaptation to stress.
CitationThe Arabidopsis RNA-Binding Protein AtRGGA Regulates Tolerance to Salt and Drought Stress 2015, 168 (1):292 Plant Physiology
- A stress-responsive caleosin-like protein, AtCLO4, acts as a negative regulator of ABA responses in Arabidopsis.
- Authors: Kim YY, Jung KW, Yoo KS, Jeung JU, Shin JS
- Issue date: 2011 May
- A Nucleus-Localized Long Non-Coding RNA Enhances Drought and Salt Stress Tolerance.
- Authors: Qin T, Zhao H, Cui P, Albesher N, Xiong L
- Issue date: 2017 Nov
- miR394 and LCR are involved in Arabidopsis salt and drought stress responses in an abscisic acid-dependent manner.
- Authors: Song JB, Gao S, Sun D, Li H, Shu XX, Yang ZM
- Issue date: 2013 Dec 11
- The tumor necrosis factor receptor-associated factor (TRAF)-like family protein SEVEN IN ABSENTIA 2 (SINA2) promotes drought tolerance in an ABA-dependent manner in Arabidopsis.
- Authors: Bao Y, Wang C, Jiang C, Pan J, Zhang G, Liu H, Zhang H
- Issue date: 2014 Apr
- AtCPL5, a novel Ser-2-specific RNA polymerase II C-terminal domain phosphatase, positively regulates ABA and drought responses in Arabidopsis.
- Authors: Jin YM, Jung J, Jeon H, Won SY, Feng Y, Kang JS, Lee SY, Cheong JJ, Koiwa H, Kim M
- Issue date: 2011 Apr