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dc.contributor.authorNtui, Valentine Otang
dc.contributor.authorKong, Kynet
dc.contributor.authorKhan, Raham Sher
dc.contributor.authorIgawa, Tomoko
dc.contributor.authorJanavi, Gnanaguru Janaky
dc.contributor.authorRabindran, Ramalingam
dc.contributor.authorNakamura, Ikuo
dc.contributor.authorMii, Masahiro
dc.date.accessioned2015-06-24T14:21:02Z
dc.date.available2015-06-24T14:21:02Z
dc.date.issued2015-04-22
dc.identifier.citationResistance to Sri Lankan Cassava Mosaic Virus (SLCMV) in Genetically Engineered Cassava cv. KU50 through RNA Silencing 2015, 10 (4):e0120551 PLOS ONE
dc.identifier.issn1932-6203
dc.identifier.pmid25901740
dc.identifier.doi10.1371/journal.pone.0120551
dc.identifier.urihttp://hdl.handle.net/10754/558487
dc.description.abstractCassava ranks fifth among the starch producing crops of the world, its annual bioethanol yield is higher than for any other crop. Cassava cultivar KU50, the most widely grown cultivar for non-food purposes is susceptible to Sri Lankan cassava mosaic virus (SLCMV). The objective of this work was to engineer resistance to SLCMV by RNA interference (RNAi) in order to increase biomass yield, an important aspect for bioethanol production. Here, we produced transgenic KU50 lines expressing dsRNA homologous to the region between the AV2 and AV1 of DNA A of SLCMV. High level expression of dsRNA of SLCMV did not induce any growth abnormality in the transgenic plants. Transgenic lines displayed high levels of resistance to SLCMV compared to the wild-type plants and no virus load could be detected in uninoculated new leaves of the infected resistant lines after PCR amplification and RT-PCR analysis. The agronomic performance of the transgenic lines was unimpaired after inoculation with the virus as the plants presented similar growth when compared to the mock inoculated control plants and revealed no apparent reduction in the amount and weight of tubers produced. We show that the resistance is correlated with post-transcriptional gene silencing because of the production of transgene specific siRNA. The results demonstrate that transgenic lines exhibited high levels of resistance to SLCMV. This resistance coupled with the desirable yield components in the transgenic lines makes them better candidates for exploitation in the production of biomass as well as bioethanol.
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0120551
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/
dc.titleResistance to Sri Lankan Cassava Mosaic Virus (SLCMV) in Genetically Engineered Cassava cv. KU50 through RNA Silencing
dc.typeArticle
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC4406713
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionLaboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, Chiba, Japan
dc.contributor.institutionDepartment of Genetics/Biotechnology, Faculty of Science, University of Calabar, Calabar, Nigeria
dc.contributor.institutionCambodia Agricultural Research and Development Institute, Phnom Penh, Cambodia
dc.contributor.institutionDepartment of Biotechnology, Abdul Wali Khan University, Mardan, Pakistan
dc.contributor.institutionHorticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore, India
kaust.personNtui, Valentine Otang
refterms.dateFOA2018-06-14T08:00:18Z


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