Compositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofouling
Emwas, Abdul-Hamid M.
Amy, Gary L.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Imaging and Characterization Core Lab
Water Desalination and Reuse Research Center (WDRC)
Online Publication Date2015-06-14
Print Publication Date2015-08
Permanent link to this recordhttp://hdl.handle.net/10754/557006
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AbstractTransparent-exopolymer-particles (TEP) have been recently identified as a significant contributor to surface biofouling, such as on reverse osmosis (RO) membranes. TEP research has mainly focused on algal TEP/TEP precursors while limited investigations have been conducted on those released by bacteria. In this study, TEP/TEP precursors derived from both algae and bacteria were isolated and then characterized to investigate their similarities and/or differences using various advanced analytical techniques, thus providing a better understanding of their potential effect on biofouling. Bacterial TEP/TEP precursors were isolated from two species of marine bacteria (Pseudidiomarina homiensis and Pseudoalteromonas atlantica) while algal TEP/TEP precursors were isolated from two marine algae species (Alexandrium tamarense and Chaetoceros affinis). Results indicated that both isolated bacterial and algal TEP/TEP precursors were associated with protein-like materials, and most TEP precursors were high-molecular-weight biopolymers. Furthermore all investigated algal and bacterial TEP/TEP precursors showed a lectin-like property, which can enable them to act as a chemical conditioning layer and to agglutinate bacteria. This property may enhance surface biofouling. However, both proton nuclear magnetic resonance (NMR) spectra and the nitrogen/carbon (N/C) ratios suggested that the algal TEP/TEP precursors contained much less protein content than the bacterial TEP/TEP precursors. This difference may influence their initial deposition and further development of surface biofouling.
CitationCompositional Similarities and Differences between Transparent Exopolymer Particles (TEP) from two Marine Bacteria and two Marine Algae: Significance to Surface Biofouling 2015 Marine Chemistry