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dc.contributor.authorPhelan, Jody
dc.contributor.authorMaitra, Arundhati
dc.contributor.authorMcNerney, Ruth
dc.contributor.authorNair, Mridul
dc.contributor.authorGupta, Antima
dc.contributor.authorColl, Francesc
dc.contributor.authorPain, Arnab
dc.contributor.authorBhakta, Sanjib
dc.contributor.authorClark, Taane G.
dc.date.accessioned2015-06-14T13:16:22Z
dc.date.available2015-06-14T13:16:22Z
dc.date.issued2015-06-05
dc.identifier.citationThe draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae 2015 International Journal of Mycobacteriology
dc.identifier.issn22125531
dc.identifier.doi10.1016/j.ijmyco.2015.05.001
dc.identifier.urihttp://hdl.handle.net/10754/556900
dc.description.abstractMycobacterium aurum (M. aurum) is an environmental mycobacteria that has previously been used in studies of anti-mycobacterial drugs due to its fast growth rate and low pathogenicity. The M. aurum genome has been sequenced and assembled into 46 contigs, with a total length of 6.02 Mb containing 5684 annotated protein-coding genes. A phylogenetic analysis using whole genome alignments positioned M. aurum close to Mycobacterium vaccae and Mycobacterium vanbaalenii, within a clade related to fast-growing mycobacteria. Large-scale genomic rearrangements were identified by comparing the M. aurum genome to those of Mycobacterium tuberculosis and Mycobacterium leprae. M. aurum orthologous genes implicated in resistance to anti-tuberculosis drugs in M. tuberculosis were observed. The sequence identity at the DNA level varied from 68.6% for pncA (pyrazinamide drug-related) to 96.2% for rrs (streptomycin, capreomycin). We observed two homologous genes encoding the catalase-peroxidase enzyme (katG) that is associated with resistance to isoniazid. Similarly, two embB homologues were identified in the M. aurum genome. In addition to describing for the first time the genome of M. aurum, this work provides a resource to aid the use of M. aurum in studies to develop improved drugs for the pathogenic mycobacteria M. tuberculosis and M. leprae.
dc.publisherMedknow
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S2212553115000783
dc.rightsArchived with thanks to International Journal of Mycobacteriology, Under a Creative Commons license, http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectGenome
dc.subjectDrug screening
dc.subjectM. leprae
dc.subjectM. tuberculosis
dc.subjectM. aurum
dc.subjectMycobacteria
dc.titleThe draft genome of Mycobacterium aurum, a potential model organism for investigating drugs against Mycobacterium tuberculosis and Mycobacterium leprae
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalInternational Journal of Mycobacteriology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionFaculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
dc.contributor.institutionMycobacteria Research Laboratory, Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdom
dc.contributor.institutionFaculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT, United Kingdom
kaust.personNair, Mridul
kaust.personPain, Arnab
refterms.dateFOA2018-06-14T07:10:06Z
dc.date.published-online2015-06-05
dc.date.published-print2015-09


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