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dc.contributor.authorWang, Zhenyu
dc.contributor.authorGehring, Christoph A
dc.contributor.authorZhu, Jianhua
dc.contributor.authorLi, Feng-Min
dc.contributor.authorZhu, Jian-Kang
dc.contributor.authorXiong, Liming
dc.date.accessioned2015-06-14T13:13:41Z
dc.date.available2015-06-14T13:13:41Z
dc.date.issued2014-11-21
dc.identifier.citationThe Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis 2015, 167 (1):137 Plant Physiology
dc.identifier.issn0032-0889
dc.identifier.issn1532-2548
dc.identifier.pmid25416474
dc.identifier.doi10.1104/pp.114.249268
dc.identifier.urihttp://hdl.handle.net/10754/556897
dc.description.abstractOsmotic stress activates the biosynthesis of the phytohormone abscisic acid (ABA) through a pathway that is rate limited by the carotenoid cleavage enzyme 9-cis-epoxycarotenoid dioxygenase (NCED). To understand the signal transduction mechanism underlying the activation of ABA biosynthesis, we performed a forward genetic screen to isolate mutants defective in osmotic stress regulation of the NCED3 gene. Here, we identified the Arabidopsis (Arabidopsis thaliana) Vacuolar Sorting Receptor1 (VSR1) as a unique regulator of ABA biosynthesis. The vsr1 mutant not only shows increased sensitivity to osmotic stress, but also is defective in the feedback regulation of ABA biosynthesis by ABA. Further analysis revealed that vacuolar trafficking mediated by VSR1 is required for osmotic stress-responsive ABA biosynthesis and osmotic stress tolerance. Moreover, under osmotic stress conditions, the membrane potential, calcium flux, and vacuolar pH changes in the vsr1 mutant differ from those in the wild type. Given that manipulation of the intracellular pH is sufficient to modulate the expression of ABA biosynthesis genes, including NCED3, and ABA accumulation, we propose that intracellular pH changes caused by osmotic stress may play a signaling role in regulating ABA biosynthesis and that this regulation is dependent on functional VSR1.
dc.publisherAmerican Society of Plant Biologists (ASPB)
dc.relation.urlhttp://www.plantphysiol.org/lookup/doi/10.1104/pp.114.249268
dc.rightsArchived with thanks to Plant Physiology
dc.titleThe Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentMolecular Signalling Group
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalPlant Physiology
dc.identifier.pmcidPMC4281004
dc.eprint.versionPost-print
dc.contributor.institutionState Key Laboratory of Grassland Agroecosystem, Institute of Arid Agroecology, School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, China
dc.contributor.institutionDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park, Maryland 20742
dc.contributor.institutionDepartment of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47907
dc.contributor.institutionShanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
kaust.personWang, Zhenyu
kaust.personGehring, Christoph A.
kaust.personXiong, Liming
refterms.dateFOA2015-11-21T00:00:00Z
dc.date.published-online2014-11-21
dc.date.published-print2015-01


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