The Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis
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Plant Physiol. 167(1) 137-52, Figures.ppt
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Type
ArticleKAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Molecular Signalling Group
Plant Science
Plant Stress Genomics Research Lab
Date
2014-11-21Online Publication Date
2014-11-21Print Publication Date
2015-01Permanent link to this record
http://hdl.handle.net/10754/556897
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Osmotic stress activates the biosynthesis of the phytohormone abscisic acid (ABA) through a pathway that is rate limited by the carotenoid cleavage enzyme 9-cis-epoxycarotenoid dioxygenase (NCED). To understand the signal transduction mechanism underlying the activation of ABA biosynthesis, we performed a forward genetic screen to isolate mutants defective in osmotic stress regulation of the NCED3 gene. Here, we identified the Arabidopsis (Arabidopsis thaliana) Vacuolar Sorting Receptor1 (VSR1) as a unique regulator of ABA biosynthesis. The vsr1 mutant not only shows increased sensitivity to osmotic stress, but also is defective in the feedback regulation of ABA biosynthesis by ABA. Further analysis revealed that vacuolar trafficking mediated by VSR1 is required for osmotic stress-responsive ABA biosynthesis and osmotic stress tolerance. Moreover, under osmotic stress conditions, the membrane potential, calcium flux, and vacuolar pH changes in the vsr1 mutant differ from those in the wild type. Given that manipulation of the intracellular pH is sufficient to modulate the expression of ABA biosynthesis genes, including NCED3, and ABA accumulation, we propose that intracellular pH changes caused by osmotic stress may play a signaling role in regulating ABA biosynthesis and that this regulation is dependent on functional VSR1.Citation
The Arabidopsis Vacuolar Sorting Receptor1 Is Required for Osmotic Stress-Induced Abscisic Acid Biosynthesis 2015, 167 (1):137 Plant PhysiologyJournal
Plant PhysiologyPubMed ID
25416474PubMed Central ID
PMC4281004Additional Links
http://www.plantphysiol.org/lookup/doi/10.1104/pp.114.249268Relations
Is Supplemented By:- [Bioproject]
Title: Expression data of WT (NCED3pro:LUC transgenic plants, gl1 Columbia background) and ced2 under osmotic stressPublication Date: 2015-02-20. bioproject: PRJNA189940 Handle: 10754/666423
ae974a485f413a2113503eed53cd6c53
10.1104/pp.114.249268
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