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dc.contributor.authorBayer, Kristina
dc.contributor.authorMoitinho-Silva, Lucas
dc.contributor.authorBrümmer, Franz
dc.contributor.authorCannistraci, Carlo
dc.contributor.authorRavasi, Timothy
dc.contributor.authorHentschel, Ute
dc.date.accessioned2015-06-07T21:27:17Z
dc.date.available2015-06-07T21:27:17Z
dc.date.issued2014-11-10
dc.identifier.citationGeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater 2014, 90 (3):832 FEMS Microbiology Ecology
dc.identifier.issn01686496
dc.identifier.doi10.1111/1574-6941.12441
dc.identifier.urihttp://hdl.handle.net/10754/556514
dc.description.abstractThe GeoChip 4.2 gene array was employed to interrogate the microbial functional gene repertoire of sponges and seawater collected from the Red Sea and the Mediterranean. Complementary amplicon sequencing confirmed the microbial community composition characteristic of high microbial abundance (HMA) and low microbial abundance (LMA) sponges. By use of GeoChip, altogether 20 273 probes encoding for 627 functional genes and representing 16 gene categories were identified. Minimum curvilinear embedding analyses revealed a clear separation between the samples. The HMA/LMA dichotomy was stronger than any possible geographic pattern, which is shown here for the first time on the level of functional genes. However, upon inspection of individual genes, very few specific differences were discernible. Differences were related to microbial ammonia oxidation, ammonification, and archaeal autotrophic carbon fixation (higher gene abundance in sponges over seawater) as well as denitrification and radiation-stress-related genes (lower gene abundance in sponges over seawater). Except for few documented specific differences the functional gene repertoire between the different sources appeared largely similar. This study expands previous reports in that functional gene convergence is not only reported between HMA and LMA sponges but also between sponges and seawater.
dc.publisherOxford University Press (OUP)
dc.relation.urlhttp://femsec.oxfordjournals.org/cgi/doi/10.1111/1574-6941.12441
dc.rightsArchived with thanks to FEMS Microbiology Ecology
dc.subjectGeoChip
dc.subjectamplicon sequencing
dc.subjectmarine sponge
dc.subjectPorifera
dc.subjectfunctional gene
dc.subjectsymbiosis
dc.titleGeoChip-based insights into the microbial functional gene repertoire of marine sponges (high microbial abundance, low microbial abundance) and seawater
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalFEMS Microbiology Ecology
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Botany II; Julius-von-Sachs Institute for Biological Sciences; University of Wuerzburg; Wuerzburg Germany
dc.contributor.institutionDepartment of Botany II; Julius-von-Sachs Institute for Biological Sciences; University of Wuerzburg; Wuerzburg Germany
dc.contributor.institutionDepartment of Zoology; Biological Institute; University of Stuttgart; Stuttgart Germany
dc.contributor.institutionDepartment of Botany II; Julius-von-Sachs Institute for Biological Sciences; University of Wuerzburg; Wuerzburg Germany
dc.contributor.institutionBiomedical Cybernetics Group, Biotechnology Center (BIOTEC), Technical University Dresden, Dresden, Germany
kaust.personCannistraci, Carlo
kaust.personRavasi, Timothy
refterms.dateFOA2016-01-08T00:00:00Z
dc.date.published-online2014-11-10
dc.date.published-print2014-12


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