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dc.contributor.authorByrt, Caitlin Siobhan
dc.contributor.authorXu, Bo
dc.contributor.authorKrishnan, Mahima
dc.contributor.authorLightfoot, Damien
dc.contributor.authorAthman, Asmini
dc.contributor.authorJacobs, Andrew Keith
dc.contributor.authorWatson-Haigh, Nathan S.
dc.contributor.authorPlett, Darren
dc.contributor.authorMunns, Rana
dc.contributor.authorTester, Mark A.
dc.contributor.authorGilliham, Matthew
dc.date.accessioned2015-06-03T08:38:14Z
dc.date.available2015-06-03T08:38:14Z
dc.date.issued2014-10-01
dc.identifier.citationThe Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat 2014, 80 (3):516 The Plant Journal
dc.identifier.issn09607412
dc.identifier.doi10.1111/tpj.12651
dc.identifier.urihttp://hdl.handle.net/10754/556197
dc.description.abstractBread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K+/Na+ ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we decipher the function of TaHKT1;5-D, a candidate gene underlying the Kna1 locus. Transport studies using the heterologous expression systems Saccharomyces cerevisiae and Xenopus laevis oocytes indicated that TaHKT1;5-D is a Na+-selective transporter. Transient expression in Arabidopsis thaliana mesophyll protoplasts and in situ polymerase chain reaction indicated that TaHKT1;5-D is localised on the plasma membrane in the wheat root stele. RNA interference-induced silencing decreased the expression of TaHKT1;5-D in transgenic bread wheat lines which led to an increase in the Na+ concentration in the leaves. This indicates that TaHKT1;5-D retrieves Na+ from the xylem vessels in the root and has an important role in restricting the transport of Na+ from the root to the leaves in bread wheat. Thus, TaHKT1;5-D confers the essential salinity tolerance mechanism in bread wheat associated with the Kna1 locus via shoot Na+ exclusion and is critical in maintaining a high K+/Na+ ratio in the leaves. These findings show there is potential to increase the salinity tolerance of bread wheat by manipulation of HKT1;5 genes.
dc.publisherWiley
dc.relation.urlhttp://doi.wiley.com/10.1111/tpj.12651
dc.rightsThis is the peer reviewed version of the following article: Byrt, C. S., Xu, B., Krishnan, M., Lightfoot, D. J., Athman, A., Jacobs, A. K., Watson-Haigh, N. S., Plett, D., Munns, R., Tester, M. and Gilliham, M. (2014), The Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat. The Plant Journal, 80: 516–526. doi: 10.1111/tpj.12651, which has been published in final form at http://doi.wiley.com/10.1111/tpj.12651. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
dc.subjectwheat
dc.subjectTriticum aestivum
dc.subjectsodium exclusion
dc.subjectHKT
dc.subjectsalt tolerance
dc.subjectRNA interference
dc.titleThe Na+ transporter, TaHKT1;5-D, limits shoot Na+ accumulation in bread wheat
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.contributor.departmentThe Salt Lab
dc.identifier.journalThe Plant Journal
dc.eprint.versionPost-print
dc.contributor.institutionCSIRO Plant Industry; Canberra ACT 2601 Australia
dc.contributor.institutionSchool of Agriculture; Food and Wine and Waite Research Institute; University of Adelaide; Waite Research Precinct; Glen Osmond SA 5064 Australia
dc.contributor.institutionAustralian Centre for Plant Functional Genomics; University of Adelaide; Waite Research Precinct; Glen Osmond SA 5064 Australia
dc.contributor.institutionAustralian Research Council Centre of Excellence in Plant Cell Walls, University of Adelaide, Waite Research Precinct, Glen Osmond, SA, Australia
dc.contributor.institutionAustralian Research Council Centre of Excellence in Plant Energy Biology, University of Adelaide, Waite Research Precinct, Glen Osmond, SA, Australia
dc.contributor.institutionSchool of Information Technology and Mathematical Sciences, University of South Australia, Mawson Lakes, SA, Australia
dc.contributor.institutionSchool of Plant Biology and ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA, Australia
kaust.personLightfoot, Damien
kaust.personTester, Mark A.
refterms.dateFOA2015-10-01T00:00:00Z
dc.date.published-online2014-10-01
dc.date.published-print2014-11


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