Rapid determination of anti-tuberculosis drug resistance from whole-genome sequences
Type
ArticleAuthors
Coll, Francesc
McNerney, Ruth

Preston, Mark D
Guerra-Assunção, José Afonso
Warry, Andrew
Hill-Cawthorne, Grant A.

Mallard, Kim
Nair, Mridul
Miranda, Anabela
Alves, Adriana
Perdigão, João

Viveiros, Miguel

Portugal, Isabel
Hasan, Zahra

Hasan, Rumina
Glynn, Judith R.

Martin, Nigel
Pain, Arnab

Clark, Taane G.

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Computational Bioscience Research Center (CBRC)
Pathogen Genomics Laboratory
Date
2015-05-27Online Publication Date
2015-05-27Print Publication Date
2015-12Permanent link to this record
http://hdl.handle.net/10754/556149
Metadata
Show full item recordAbstract
Mycobacterium tuberculosis drug resistance (DR) challenges effective tuberculosis disease control. Current molecular tests examine limited numbers of mutations, and although whole genome sequencing approaches could fully characterise DR, data complexity has restricted their clinical application. A library (1,325 mutations) predictive of DR for 15 anti-tuberculosis drugs was compiled and validated for 11 of them using genomic-phenotypic data from 792 strains. A rapid online ‘TB-Profiler’ tool was developed to report DR and strain-type profiles directly from raw sequences. Using our DR mutation library, in silico diagnostic accuracy was superior to some commercial diagnostics and alternative databases. The library will facilitate sequence-based drug-susceptibility testing.Citation
Rapid determination of anti-tuberculosis drug resistance from whole-genome sequences 2015, 7 (1) Genome MedicinePublisher
Springer NatureJournal
Genome MedicinePubMed ID
26019726PubMed Central ID
PMC4446134Additional Links
http://genomemedicine.com/content/7/1/51ae974a485f413a2113503eed53cd6c53
10.1186/s13073-015-0164-0
Scopus Count
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