Rapid determination of anti-tuberculosis drug resistance from whole-genome sequences
Preston, Mark D
Guerra-Assunção, José Afonso
Hill-Cawthorne, Grant A.
Glynn, Judith R.
Clark, Taane G.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Pathogen Genomics Laboratory
Online Publication Date2015-05-27
Print Publication Date2015-12
Permanent link to this recordhttp://hdl.handle.net/10754/556149
MetadataShow full item record
AbstractMycobacterium tuberculosis drug resistance (DR) challenges effective tuberculosis disease control. Current molecular tests examine limited numbers of mutations, and although whole genome sequencing approaches could fully characterise DR, data complexity has restricted their clinical application. A library (1,325 mutations) predictive of DR for 15 anti-tuberculosis drugs was compiled and validated for 11 of them using genomic-phenotypic data from 792 strains. A rapid online ‘TB-Profiler’ tool was developed to report DR and strain-type profiles directly from raw sequences. Using our DR mutation library, in silico diagnostic accuracy was superior to some commercial diagnostics and alternative databases. The library will facilitate sequence-based drug-susceptibility testing.
CitationRapid determination of anti-tuberculosis drug resistance from whole-genome sequences 2015, 7 (1) Genome Medicine
PubMed Central IDPMC4446134
- [Frontier of mycobacterium research--host vs. mycobacterium].
- Authors: Okada M, Shirakawa T
- Issue date: 2005 Sep
- [Development of antituberculous drugs: current status and future prospects].
- Authors: Tomioka H, Namba K
- Issue date: 2006 Dec
- Benefits and challenges of molecular diagnostics for childhood tuberculosis.
- Authors: Gutierrez C
- Issue date: 2016 Dec
- Best approaches to drug-resistance surveillance at the country level.
- Authors: Cabibbe AM, Cirillo DM
- Issue date: 2016 Dec
- Whole Genome Sequencing Investigation of a Tuberculosis Outbreak in Port-au-Prince, Haiti Caused by a Strain with a "Low-Level" rpoB Mutation L511P - Insights into a Mechanism of Resistance Escalation.
- Authors: Ocheretina O, Shen L, Escuyer VE, Mabou MM, Royal-Mardi G, Collins SE, Pape JW, Fitzgerald DW
- Issue date: 2015