Mechanical Model of Geometric Cell and Topological Algorithm for Cell Dynamics from Single-Cell to Formation of Monolayered Tissues with Pattern
MetadataShow full item record
AbstractGeometric and mechanical properties of individual cells and interactions among neighboring cells are the basis of formation of tissue patterns. Understanding the complex interplay of cells is essential for gaining insight into embryogenesis, tissue development, and other emerging behavior. Here we describe a cell model and an efficient geometric algorithm for studying the dynamic process of tissue formation in 2D (e.g. epithelial tissues). Our approach improves upon previous methods by incorporating properties of individual cells as well as detailed description of the dynamic growth process, with all topological changes accounted for. Cell size, shape, and division plane orientation are modeled realistically. In addition, cell birth, cell growth, cell shrinkage, cell death, cell division, cell collision, and cell rearrangements are now fully accounted for. Different models of cell-cell interactions, such as lateral inhibition during the process of growth, can be studied in detail. Cellular pattern formation for monolayered tissues from arbitrary initial conditions, including that of a single cell, can also be studied in detail. Computational efficiency is achieved through the employment of a special data structure that ensures access to neighboring cells in constant time, without additional space requirement. We have successfully generated tissues consisting of more than 20,000 cells starting from 2 cells within 1 hour. We show that our model can be used to study embryogenesis, tissue fusion, and cell apoptosis. We give detailed study of the classical developmental process of bristle formation on the epidermis of D. melanogaster and the fundamental problem of homeostatic size control in epithelial tissues. Simulation results reveal significant roles of solubility of secreted factors in both the bristle formation and the homeostatic control of tissue size. Our method can be used to study broad problems in monolayered tissue formation. Our software is publicly available.
CitationMechanical Model of Geometric Cell and Topological Algorithm for Cell Dynamics from Single-Cell to Formation of Monolayered Tissues with Pattern 2015, 10 (5):e0126484 PLOS ONE
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC4431879
- Dynamic mechanical finite element model of biological cells for studying cellular pattern formation.
- Authors: Zhao J, Naveed H, Kachalo S, Cao Y, Tian W, Liang J
- Issue date: 2013
- Geometric order in proliferating epithelia: impact of rearrangements and cleavage plane orientation.
- Authors: Naveed H, Li Y, Kachalo S, Liang J
- Issue date: 2010
- Apoptotic force and tissue dynamics during Drosophila embryogenesis.
- Authors: Toyama Y, Peralta XG, Wells AR, Kiehart DP, Edwards GS
- Issue date: 2008 Sep 19
- Mechanisms of regulating cell topology in proliferating epithelia: impact of division plane, mechanical forces, and cell memory.
- Authors: Li Y, Naveed H, Kachalo S, Xu LX, Liang J
- Issue date: 2012
- Modeling and inferring cleavage patterns in proliferating epithelia.
- Authors: Patel AB, Gibson WT, Gibson MC, Nagpal R
- Issue date: 2009 Jun