The Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells
Type
ArticleAuthors
Neumann, ChristinaFraiture, Malou
Hernàndez-Reyes, Casandra
Akum, Fidele N.
Virlogeux-Payant, Isabelle
Chen, Ying

Pateyron, Stephanie
Colcombet, Jean
Kogel, Karl-Heinz
Hirt, Heribert

Brunner, Frédéric
Schikora, Adam
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionDesert Agriculture Initiative
Plant Science
Plant Science Program
Date
2014-10-17Permanent link to this record
http://hdl.handle.net/10754/555860
Metadata
Show full item recordAbstract
Salmonella is one of the most prominent causes of food poisoning and growing evidence indicates that contaminated fruits and vegetables are an increasing concern for human health. Successful infection demands the suppression of the host immune system, which is often achieved via injection of bacterial effector proteins into host cells. In this report we present the function of Salmonella effector protein in plant cell, supporting the new concept of trans-kingdom competence of this bacterium. We screened a range of Salmonella Typhimurium effector proteins for interference with plant immunity. Among these, the phosphothreonine lyase SpvC attenuated the induction of immunity-related genes when present in plant cells. Using in vitro and in vivo systems we show that this effector protein interacts with and dephosphorylates activated Arabidopsis Mitogen-activated Protein Kinase 6 (MPK6), thereby inhibiting defense signaling. Moreover, the requirement of Salmonella SpvC was shown by the decreased proliferation of the ΔspvC mutant in Arabidopsis plants. These results suggest that some Salmonella effector proteins could have a conserved function during proliferation in different hosts. The fact that Salmonella and other Enterobacteriaceae use plants as hosts strongly suggests that plants represent a much larger reservoir for animal pathogens than so far estimated.Citation
The Salmonella effector protein SpvC, a phosphothreonine lyase is functional in plant cells 2014, 5 Frontiers in MicrobiologyPublisher
Frontiers Media SAJournal
Frontiers in Microbiologyae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2014.00548