Show simple item record

dc.contributor.authorKuo, D.
dc.contributor.authorLicon, K.
dc.contributor.authorBandyopadhyay, S.
dc.contributor.authorChuang, R.
dc.contributor.authorLUO, C.
dc.contributor.authorCatalana, J.
dc.contributor.authorRavasi, Timothy
dc.contributor.authorTan, K.
dc.contributor.authorIdeker, T.
dc.date.accessioned2015-05-26T08:04:54Z
dc.date.available2015-05-26T08:04:54Z
dc.date.issued2010-10-26
dc.identifier.citationCoevolution within a transcriptional network by compensatory trans and cis mutations 2010, 20 (12):1672 Genome Research
dc.identifier.issn1088-9051
dc.identifier.pmid20978140
dc.identifier.doi10.1101/gr.111765.110
dc.identifier.urihttp://hdl.handle.net/10754/555797
dc.description.abstractTranscriptional networks have been shown to evolve very rapidly, prompting questions as to how such changes arise and are tolerated. Recent comparisons of transcriptional networks across species have implicated variations in the cis-acting DNA sequences near genes as the main cause of divergence. What is less clear is how these changes interact with trans-acting changes occurring elsewhere in the genetic circuit. Here, we report the discovery of a system of compensatory trans and cis mutations in the yeast AP-1 transcriptional network that allows for conserved transcriptional regulation despite continued genetic change. We pinpoint a single species, the fungal pathogen Candida glabrata, in which a trans mutation has occurred very recently in a single AP-1 family member, distinguishing it from its Saccharomyces ortholog. Comparison of chromatin immunoprecipitation profiles between Candida and Saccharomyces shows that, despite their different DNA-binding domains, the AP-1 orthologs regulate a conserved block of genes. This conservation is enabled by concomitant changes in the cis-regulatory motifs upstream of each gene. Thus, both trans and cis mutations have perturbed the yeast AP-1 regulatory system in such a way as to compensate for one another. This demonstrates an example of “coevolution” between a DNA-binding transcription factor and its cis-regulatory site, reminiscent of the coevolution of protein binding partners.
dc.publisherCold Spring Harbor Laboratory
dc.relation.urlhttp://genome.cshlp.org/cgi/doi/10.1101/gr.111765.110
dc.rightsArchived with thanks to Genome Research
dc.titleCoevolution within a transcriptional network by compensatory trans and cis mutations
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalGenome Research
dc.identifier.pmcidPMC2989993
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartments of Bioengineering and Medicine, University of California, San Diego, La Jolla, California 92093, USA
dc.contributor.institutionDepartments of Internal Medicine and Biomedical Engineering, University of Iowa, Iowa City, Iowa 52242, USA
kaust.personRavasi, Timothy
refterms.dateFOA2018-06-14T06:39:23Z
dc.date.published-online2010-10-26
dc.date.published-print2010-12-01


Files in this item

Thumbnail
Name:
Genome Res.-2010-Kuo-1672-8.pdf
Size:
1.116Mb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
Supplement_YAP_Resubmission.doc
Size:
100Kb
Format:
Microsoft Word
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure1.pdf
Size:
274.9Kb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure2.pdf
Size:
316.8Kb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure3.pdf
Size:
319.8Kb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure4_2.pdf
Size:
284.3Kb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure5.pdf
Size:
258.0Kb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure6.pdf
Size:
1.292Mb
Format:
PDF
Description:
Supplemental files
Thumbnail
Name:
SupplementaryFigure7.pdf
Size:
323.2Kb
Format:
PDF
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record