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dc.contributor.authorKumar, Nitish
dc.contributor.authorVijay Anand, K. G.
dc.contributor.authorReddy, Muppala P.
dc.date.accessioned2015-05-26T08:00:28Z
dc.date.available2015-05-26T08:00:28Z
dc.date.issued2011-01-28
dc.identifier.citationPlant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants 2011, 20 (1):125 Journal of Plant Biochemistry and Biotechnology
dc.identifier.issn0971-7811
dc.identifier.issn0974-1275
dc.identifier.doi10.1007/s13562-011-0037-6
dc.identifier.urihttp://hdl.handle.net/10754/555792
dc.description.abstractJatropha curcas is an oil bearing species with multiple uses and considerable economic potential as a biofuel plant, however, oil and deoiled cake are toxic. A non-toxic variety of J. curcas is reported from Mexico. The present investigation explores the effects of different plant growth regulators (PGRs) viz. 6-benzyl aminopurine (BAP) or thidiazuron (TDZ) individually and in combination with indole-3-butyric acid (IBA), on regeneration from in vitro and field-grown mature leaf explants, in vitro and glasshouse-grown seedlings cotyledonary leaf explants of non-toxic J. curcas. In all the tested parameters maximum regeneration efficiency (81.07%) and the number of shoot buds per explants (20.17) was observed on 9.08 μM TDZ containing Murashige and Skoog’s (MS) medium from in vitro cotyledonary leaf explants. The regenerated shoot buds were transferred to MS medium containing 10 μM kinetin (Kn), 4.5 μM BAP and 5.5 μM α-naphthaleneacetic acid (NAA) for shoot proliferation. The proliferated shoots could be elongated on MS medium supplemented with 2.25 μM BAP and 8.5 μM IAA. Rooting was achieved when the basal cut end of elongated shoots were dipped in half strength MS liquid medium containing different concentrations and combinations of IBA, IAA and NAA for four days followed by transfer to growth regulators free half strength MS medium supplemented 0.25 mg/l activated charcoal. The rooted plants could be established in soil with more than 90% survival rate.
dc.publisherSpringer Nature
dc.relation.urlhttp://link.springer.com/10.1007/s13562-011-0037-6
dc.rightsArchived with thanks to Journal of Plant Biochemistry and Biotechnology
dc.titlePlant regeneration of non-toxic Jatropha curcas—impacts of plant growth regulators, source and type of explants
dc.typeArticle
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.identifier.journalJournal of Plant Biochemistry and Biotechnology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCentre for Biotechnology, School of Earth, Biological and Environmental Science, Central University of Bihar, Patna, 800014, Bihar, India
dc.contributor.institutionDiscipline of Wasteland Research, Central Salt & Marine Chemicals Research Institute (Council of Scientific and Industrial Research), Bhavnagar, Gujarat, 364002, India
kaust.personReddy, Muppala P.
refterms.dateFOA2018-06-13T11:35:07Z
dc.date.published-online2011-01-28
dc.date.published-print2011-01


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