Genome-Wide Transposon Mutagenesis Indicates that Mycobacterium marinum Customizes Its Virulence Mechanisms for Survival and Replication in Different Hosts
Name:
Infect. Immun.-2015-Weerdenburg-1778-88.pdf
Size:
3.481Mb
Format:
PDF
Description:
Main article
Name:
zii999091185so2.xlsx
Size:
1.359Mb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Type
ArticleAuthors
Weerdenburg, Eveline M.Abdallah, Abdallah
Rangkuti, Farania
Abd El Ghany, Moataz
Otto, Thomas D.
Adroub, Sabir
Molenaar, Douwe
Ummels, Roy
ter Veen, Kars
van Stempvoort, Gunny
van der Sar, Astrid M.
Ali, Shahjahan
Langridge, Gemma C.
Thomson, Nicholas R.
Pain, Arnab

Bitter, Wilbert

KAUST Department
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) DivisionComputer Science Program
Biological and Environmental Sciences and Engineering (BESE) Division
Bioscience Core Lab
Bioscience Program
Pathogen Genomics Laboratory
Computational Bioscience Research Center (CBRC)
Date
2015-02-17Online Publication Date
2015-02-17Print Publication Date
2015-05Permanent link to this record
http://hdl.handle.net/10754/552268
Metadata
Show full item recordAbstract
The interaction of environmental bacteria with unicellular eukaryotes is generally considered a major driving force for the evolution of intracellular pathogens, allowing them to survive and replicate in phagocytic cells of vertebrate hosts. To test this hypothesis on a genome-wide level, we determined for the intracellular pathogen Mycobacterium marinum whether it uses conserved strategies to exploit host cells from both protozoan and vertebrate origin. Using transposon-directed insertion site sequencing (TraDIS), we determined differences in genetic requirements for survival and replication in phagocytic cells of organisms from different kingdoms. In line with the general hypothesis, we identified a number of general virulence mechanisms, including the type VII protein secretion system ESX-1, biosynthesis of polyketide lipids, and utilization of sterols. However, we were also able to show that M. marinum contains an even larger set of host-specific virulence determinants, including proteins involved in the modification of surface glycolipids and, surprisingly, the auxiliary proteins of the ESX-1 system. Several of these factors were in fact counterproductive in other hosts. Therefore, M. marinum contains different sets of virulence factors that are tailored for specific hosts. Our data imply that although amoebae could function as a training ground for intracellular pathogens, they do not fully prepare pathogens for crossing species barriers.Citation
Genome-Wide Transposon Mutagenesis Indicates that Mycobacterium marinum Customizes Its Virulence Mechanisms for Survival and Replication in Different Hosts 2015, 83 (5):1778 Infection and ImmunityPublisher
American Society for MicrobiologyJournal
Infection and ImmunityPubMed ID
25690095PubMed Central ID
PMC4399070Additional Links
http://iai.asm.org/lookup/doi/10.1128/IAI.03050-14ae974a485f413a2113503eed53cd6c53
10.1128/IAI.03050-14
Scopus Count
Related articles
- A New ESX-1 Substrate in Mycobacterium marinum That Is Required for Hemolysis but Not Host Cell Lysis.
- Authors: Bosserman RE, Nicholson KR, Champion MM, Champion PA
- Issue date: 2019 Jul 15
- Esx Paralogs Are Functionally Equivalent to ESX-1 Proteins but Are Dispensable for Virulence in Mycobacterium marinum.
- Authors: Bosserman RE, Thompson CR, Nicholson KR, Champion PA
- Issue date: 2018 Jun 1
- A mycobacterial virulence gene cluster extending RD1 is required for cytolysis, bacterial spreading and ESAT-6 secretion.
- Authors: Gao LY, Guo S, McLaughlin B, Morisaki H, Engel JN, Brown EJ
- Issue date: 2004 Sep
- Conserved mechanisms of Mycobacterium marinum pathogenesis within the environmental amoeba Acanthamoeba castellanii.
- Authors: Kennedy GM, Morisaki JH, Champion PA
- Issue date: 2012 Mar
- Identification of Mycobacterium marinum virulence genes using signature-tagged mutagenesis and the goldfish model of mycobacterial pathogenesis.
- Authors: Ruley KM, Ansede JH, Pritchett CL, Talaat AM, Reimschuessel R, Trucksis M
- Issue date: 2004 Mar 12