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dc.contributor.authorSarhan, Abbaa
dc.contributor.authorBocca, Silvina
dc.contributor.authorYu, Liang
dc.contributor.authorAnderson, Sandra
dc.contributor.authorJacot, Terry
dc.contributor.authorBurch, Tanya
dc.contributor.authorNyalwidhe, Julius O
dc.contributor.authorSullivan, Claretta
dc.contributor.authorKaur, Mandeep
dc.contributor.authorBajic, Vladimir B.
dc.contributor.authorOehninger, Sergio
dc.date.accessioned2015-04-28T11:29:06Z
dc.date.available2015-04-28T11:29:06Z
dc.date.issued2013
dc.identifier.citationHuman endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG) 2013, 1 (1):1 Cell signalling and Trafficking
dc.identifier.issn2054-1481
dc.identifier.doi10.7243/2054-1481-1-1
dc.identifier.urihttp://hdl.handle.net/10754/550813
dc.description.abstractObjective: We have recently showed that MFGE8, a novel epithelial cell protein in the human endometrium, upregulated during the window of implantation. We hypothesized that MFGE8 may act as a key modulator of endometrial remodeling and trophoblast invasion. The aims of this study were (i) to investigate the in vitro regulation of human endometrial epithelial cells MFGE8 transcription, translation, and secretion by sex steroids and hCG; and (ii) to examine the possibility of MFGE8 secretion via microvesicles. Design: Experimental in vitro study using Ishikawa cells. Setting: University center. Interventions: Treatment with estradiol (E2), progesterone (P4), and human chorionic gonatropin (hCG). Main outcome measures: MFGE8 mRNA and protein expression, and identification of secreted microvesicles by mass spectrometry (MS) and immunoblotting. Results: E2, but not P4 or hCG, significantly upregulated MFGE8 mRNA expression. hCG significantly increased MFGE8 secretion. Microvesicels obtained after ultracentrifugation were visualized with atomic force microscopy ranging from ~100 to 200 nm. In addition to the expected 46 kD protein, the microvesicles contained a second form of secreted MFGE8 measuring ~30 kD which was confirmed by MS. Conclusions: We demonstrated (i) dual effects of E2 and hCG on the regulation of MFGE8, and (ii) MFGE8 protein secretion in association with microvesicles. MFGE8 has the potential to modulate endometrial function and implantation via exocrine and/ or paracrine-autocrine effects. To the best of our knowledge, this is the first demonstration of microvesicular secretion of any regulatory protein by endometrial epithelial cells, providing initial evidence suggestive of microvesicular participation in cellular trafficking information in the non-pregnant and pregnant endometrium.
dc.publisherHerbert Publications PVT LTD
dc.relation.urlhttp://www.hoajonline.com/cellsignaltraffic/2054-1481/1/1
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subjectEndometrium
dc.subjectgene expression
dc.subjectMFGE8
dc.subjectmicrovesicles
dc.titleHuman endometrial milk fat globule-epidermal growth factor 8 (MFGE8) is up regulated by estradiol at the transcriptional level, and its secretion via microvesicles is stimulated by human chorionic gonadotropin (hCG)
dc.typeArticle
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentApplied Mathematics and Computational Science Program
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
dc.identifier.journalCell signalling and Trafficking
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionThe Jones institute for Reproductive Medicine, Department of Obstetrics and Gynecology, USA
dc.contributor.institutionDepartment of Microbiology and Molecular Cell Biology -Center for Biomedical Proteomics, USA
dc.contributor.institutionDepartment of Surgery, Eastern Virginia Medical School, Norfolk, Virginia 23507, USA
dc.contributor.institutionThe Jones institute for Reproductive Medicine, Eastern Virginia Medical School, Department of Obstetrics and Gynecology, USA
dc.contributor.institutionColumbia Fertility Associates, 2440 M St NW, Suite 401, Washington DC 20037, USA
kaust.personKaur, Mandeep
kaust.personBajic, Vladimir B.
refterms.dateFOA2018-06-13T17:18:53Z


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