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dc.contributor.authorMojib, Nazia
dc.contributor.authorAmad, Maan H.
dc.contributor.authorThimma, Manjula
dc.contributor.authorAldanondo, Naroa
dc.contributor.authorMande, Kumaran Kuppusamy
dc.contributor.authorIrigoien, Xabier
dc.date.accessioned2015-04-28T08:45:29Z
dc.date.available2015-04-28T08:45:29Z
dc.date.issued2014-05-22
dc.identifier.citationCarotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia 2014, 23 (11):2740 Molecular Ecology
dc.identifier.issn09621083
dc.identifier.pmid24803335
dc.identifier.doi10.1111/mec.12781
dc.identifier.urihttp://hdl.handle.net/10754/550807
dc.description.abstractThe tropical oligotrophic oceanic areas are characterized by high water transparency and annual solar radiation. Under these conditions, a large number of phylogenetically diverse mesozooplankton species living in the surface waters (neuston) are found to be blue pigmented. In the present study, we focused on understanding the metabolic and genetic basis of the observed blue phenotype functional equivalence between the blue-pigmented organisms from the phylum Arthropoda, subclass Copepoda (Acartia fossae) and the phylum Chordata, class Appendicularia (Oikopleura dioica) in the Red Sea. Previous studies have shown that carotenoid–protein complexes are responsible for blue coloration in crustaceans. Therefore, we performed carotenoid metabolic profiling using both targeted and nontargeted (high-resolution mass spectrometry) approaches in four different blue-pigmented genera of copepods and one blue-pigmented species of appendicularia. Astaxanthin was found to be the principal carotenoid in all the species. The pathway analysis showed that all the species can synthesize astaxanthin from β-carotene, ingested from dietary sources, via 3-hydroxyechinenone, canthaxanthin, zeaxanthin, adonirubin or adonixanthin. Further, using de novo assembled transcriptome of blue A. fossae (subclass Copepoda), we identified highly expressed homologous β-carotene hydroxylase enzymes and putative carotenoid-binding proteins responsible for astaxanthin formation and the blue phenotype. In blue O. dioica (class Appendicularia), corresponding putative genes were identified from the reference genome. Collectively, our data provide molecular evidences for the bioconversion and accumulation of blue astaxanthin–protein complexes underpinning the observed ecological functional equivalence and adaptive convergence among neustonic mesozooplankton.
dc.publisherWiley
dc.relation.urlhttp://doi.wiley.com/10.1111/mec.12781
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.subjectastaxanthin
dc.subjectcarotenoid-binding proteins
dc.subjecthigh-resolution mass spectrometry
dc.subjectmesozooplankton
dc.subjecttranscriptome
dc.subjectβ-carotene hydroxylase
dc.titleCarotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia
dc.typeArticle
dc.contributor.departmentAnalytical Chemistry Core Lab
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentEngagement
dc.contributor.departmentEnvironmental
dc.contributor.departmentMarine Science Program
dc.contributor.departmentPlankton ecology Research Group
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalMolecular Ecology
dc.relation.referencesMojib, N., Amad, M., Thimma, M., Aldanondo, N., Kumaran, M., & Irigoien, X. (2014). Data from: Carotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia (Version 1) [Data set]. Dryad Digital Repository. https://doi.org/10.5061/dryad.mm18b
dc.relation.referencesDOI:10.5061/DRYAD.MM18B
dc.relation.referencesHANDLE:http://hdl.handle.net/10754/624188
dc.eprint.versionPublisher's Version/PDF
kaust.personMojib, Nazia
kaust.personAmad, Maan H.
kaust.personThimma, Manjula
kaust.personAldanondo, Naroa
kaust.personIrigoien, Xabier
kaust.personMande, Kumaran Kuppusamy
refterms.dateFOA2018-06-13T17:07:55Z
dc.date.published-online2014-05-22
dc.date.published-print2014-06


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