Carotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia
Name:
Mojib_et_al-2014-Molecular_Ecology.pdf
Size:
3.003Mb
Format:
PDF
Description:
Main article
Name:
mec12781-sup-0001-FigS1-S4.pdf
Size:
1.013Mb
Format:
PDF
Description:
Supplemental files
Name:
mec12781-sup-0002-TableS1.pdf
Size:
60.68Kb
Format:
PDF
Description:
Supplemental files
Name:
mec12781-sup-0003-TableS2.xlsx
Size:
30.48Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Name:
mec12781-sup-0004-TableS3.xlsx
Size:
21.25Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Name:
mec12781-sup-0005-TableS4.xlsx
Size:
19.93Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Name:
mec12781-sup-0006-AppendixS1.pdf
Size:
9.100Kb
Format:
PDF
Description:
Supplemental files
Name:
mec12781-sup-0007-AppendixS2.pdb
Size:
127.3Kb
Format:
Unknown
Description:
Supplemental files
Name:
mec12781-sup-0008-AppendixS3.pdb
Size:
123.5Kb
Format:
Unknown
Description:
Supplemental files
Name:
mec12781-sup-0009-AppendixS4.pdb
Size:
120.8Kb
Format:
Unknown
Description:
Supplemental files
Type
ArticleAuthors
Mojib, Nazia
Amad, Maan H.
Thimma, Manjula
Aldanondo, Naroa
Mande, Kumaran Kuppusamy
Irigoien, Xabier

KAUST Department
Analytical Chemistry Core LabBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Core Lab
Engagement
Environmental
Marine Science Program
Plankton ecology Research Group
Red Sea Research Center (RSRC)
Date
2014-05-22Online Publication Date
2014-05-22Print Publication Date
2014-06Permanent link to this record
http://hdl.handle.net/10754/550807
Metadata
Show full item recordAbstract
The tropical oligotrophic oceanic areas are characterized by high water transparency and annual solar radiation. Under these conditions, a large number of phylogenetically diverse mesozooplankton species living in the surface waters (neuston) are found to be blue pigmented. In the present study, we focused on understanding the metabolic and genetic basis of the observed blue phenotype functional equivalence between the blue-pigmented organisms from the phylum Arthropoda, subclass Copepoda (Acartia fossae) and the phylum Chordata, class Appendicularia (Oikopleura dioica) in the Red Sea. Previous studies have shown that carotenoid–protein complexes are responsible for blue coloration in crustaceans. Therefore, we performed carotenoid metabolic profiling using both targeted and nontargeted (high-resolution mass spectrometry) approaches in four different blue-pigmented genera of copepods and one blue-pigmented species of appendicularia. Astaxanthin was found to be the principal carotenoid in all the species. The pathway analysis showed that all the species can synthesize astaxanthin from β-carotene, ingested from dietary sources, via 3-hydroxyechinenone, canthaxanthin, zeaxanthin, adonirubin or adonixanthin. Further, using de novo assembled transcriptome of blue A. fossae (subclass Copepoda), we identified highly expressed homologous β-carotene hydroxylase enzymes and putative carotenoid-binding proteins responsible for astaxanthin formation and the blue phenotype. In blue O. dioica (class Appendicularia), corresponding putative genes were identified from the reference genome. Collectively, our data provide molecular evidences for the bioconversion and accumulation of blue astaxanthin–protein complexes underpinning the observed ecological functional equivalence and adaptive convergence among neustonic mesozooplankton.Citation
Carotenoid metabolic profiling and transcriptome-genome mining reveal functional equivalence among blue-pigmented copepods and appendicularia 2014, 23 (11):2740 Molecular EcologyPublisher
WileyJournal
Molecular EcologyPubMed ID
24803335Additional Links
http://doi.wiley.com/10.1111/mec.12781ae974a485f413a2113503eed53cd6c53
10.1111/mec.12781
Scopus Count
Related articles
- Characterization of cyanobacterial carotenoid ketolase CrtW and hydroxylase CrtR by complementation analysis in Escherichia coli.
- Authors: Makino T, Harada H, Ikenaga H, Matsuda S, Takaichi S, Shindo K, Sandmann G, Ogata T, Misawa N
- Issue date: 2008 Dec
- A carotenoid synthesis gene cluster from a non-marine Brevundimonas that synthesizes hydroxylated astaxanthin.
- Authors: Tao L, Rouvière PE, Cheng Q
- Issue date: 2006 Sep 1
- A novel method for screening a vertebrate transcriptome for genes involved in carotenoid binding and metabolism.
- Authors: Pointer MA, Prager M, Andersson S, Mundy NI
- Issue date: 2012 Jan
- The crtS gene of Xanthophyllomyces dendrorhous encodes a novel cytochrome-P450 hydroxylase involved in the conversion of beta-carotene into astaxanthin and other xanthophylls.
- Authors: Alvarez V, Rodríguez-Sáiz M, de la Fuente JL, Gudiña EJ, Godio RP, Martín JF, Barredo JL
- Issue date: 2006 Apr
- Mutational and functional analysis of the beta-carotene ketolase involved in the production of canthaxanthin and astaxanthin.
- Authors: Ye RW, Stead KJ, Yao H, He H
- Issue date: 2006 Sep