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dc.contributor.authorRaji, Misjudeen
dc.contributor.authorGehring, Christoph A
dc.contributor.authorSharkhuu, Altanbadralt
dc.contributor.authorAmad, Maan H.
dc.contributor.authorMerzaban, Jasmeen
dc.date.accessioned2015-04-27T16:33:56Z
dc.date.available2015-04-27T16:33:56Z
dc.date.issued2014
dc.identifier.citationDetermination of Inter-leaf Translocated Free Glyphosate in Arabidopsis thaliana using Liquid Chromatography Tandem Mass Spectrometry (LCMS/MS) after Derivatization with Fluorenylmethyloxycarbonyl Chloride (FMOC-Cl) 2014, s2 (01) Journal of Analytical & Bioanalytical Techniques
dc.identifier.issn21559872
dc.identifier.doi10.4172/2155-9872.S2-007
dc.identifier.urihttp://hdl.handle.net/10754/550727
dc.description.abstractGlyphosate is a broad-spectrum herbicide widely used for eliminating weeds in crop fields. Its mode of action is believed to be via translocation from the source to the sink tissues where it then interferes with the activities of 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS). In this study, the translocation of glyphosate in the leaves of Arabidopsis thaliana was investigated using an HPLC-MS/MS method following derivatization of the secondary amino group in the analyte using N-(9-fluorenylmethoxycarbonyloxy) chloride. To eliminate the errant precipitation that occurred when the reagent and the analyte are mixed, optimization of this method was required. The method linearity has a correlation coefficient higher than 0.99 over the concentration range of 0.005-2 μM. The limits of detection and quantitation were estimated to be 0.002 μM and 0.008 μM respectively. The repeatability of the method (as%R.S.D) ranged from 10% to 13%. The presented method was employed for the determination of free glyphosate in young untreated leaves of the specimen plants after treating a single leaf and allowing it to stand for 12 hours.
dc.publisherOMICS Publishing Group
dc.relation.urlhttp://www.omicsonline.org/open-access/determination-of-inter-leaf-translocated-free-glyphosate-in-arabidopsis-thaliana-2155-9872.S2-007.php?aid=24120
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.titleDetermination of Inter-leaf Translocated Free Glyphosate in Arabidopsis thaliana using Liquid Chromatography Tandem Mass Spectrometry (LCMS/MS) after Derivatization with Fluorenylmethyloxycarbonyl Chloride (FMOC-Cl)
dc.typeArticle
dc.contributor.departmentAnalytical Chemistry Core Lab
dc.contributor.departmentAnalytical Core Lab
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentEngagement
dc.contributor.departmentEnvironmental
dc.contributor.departmentMolecular Signalling Group
dc.identifier.journalJournal of Analytical & Bioanalytical Techniques
dc.eprint.versionPublisher's Version/PDF
kaust.personRaji, Misjudeen
kaust.personGehring, Christoph A.
kaust.personSharkhuu, Altanbadralt
kaust.personAmad, Maan H.
kaust.personMerzaban, Jasmeen S.
refterms.dateFOA2018-06-13T17:08:56Z


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