Comprehensive Evaluation of Toxoplasma gondii VEG and Neospora caninum LIV Genomes with Tachyzoite Stage Transcriptome and Proteome Defines Novel Transcript Features
Name:
S1_Fig.tif
Size:
872.3Kb
Format:
TIFF image
Description:
Supplemental files
Name:
S1_Table.xlsx
Size:
38.06Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Name:
S2_Fig.tif
Size:
1.946Mb
Format:
TIFF image
Description:
Supplemental files
Name:
S2_Table.xlsx
Size:
4.984Mb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Name:
S3_Fig.tif
Size:
78.01Kb
Format:
TIFF image
Description:
Supplemental files
Name:
S3_Table.xlsx
Size:
8.546Kb
Format:
Microsoft Excel 2007
Description:
Supplemental files
Type
ArticleAuthors
Ramaprasad, Abhinay
Mourier, Tobias
Naeem, Raeece

Malas, Tareq Majed Yasin

Moussa, Ehab
Panigrahi, Aswini Kumar
Vermont, Sarah J.
Otto, Thomas D.
Wastling, Jonathan
Pain, Arnab

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Core Lab
Bioscience Program
Computer Science Program
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Pathogen Genomics Laboratory
Date
2015-04-13Permanent link to this record
http://hdl.handle.net/10754/550412
Metadata
Show full item recordAbstract
Toxoplasma gondii is an important protozoan parasite that infects all warm-blooded animals and causes opportunistic infections in immuno-compromised humans. Its closest relative, Neospora caninum, is an important veterinary pathogen that causes spontaneous abortion in livestock. Comparative genomics of these two closely related coccidians has been of particular interest to identify genes that contribute to varied host cell specificity and disease. Here, we describe a manual evaluation of these genomes based on strand-specific RNA sequencing and shotgun proteomics from the invasive tachyzoite stages of these two parasites. We have corrected predicted structures of over one third of the previously annotated gene models and have annotated untranslated regions (UTRs) in over half of the predicted protein-coding genes. We observe distinctly long UTRs in both the organisms, almost four times longer than other model eukaryotes. We have also identified a putative set of cis-natural antisense transcripts (cis-NATs) and long intergenic non-coding RNAs (lincRNAs). We have significantly improved the annotation quality in these genomes that would serve as a manually curated dataset for Toxoplasma and Neospora research communities.Citation
Ramaprasad A, Mourier T, Naeem R, Malas TB, Moussa E, et al. (2015) Comprehensive Evaluation of Toxoplasma gondii VEG and Neospora caninum LIV Genomes with Tachyzoite Stage Transcriptome and Proteome Defines Novel Transcript Features. PLoS ONE 10(4): e0124473. doi:10.1371/journal.pone.0124473Publisher
Public Library of Science (PLoS)Journal
PLoS ONEPubMed ID
25875305PubMed Central ID
PMC4395442Additional Links
http://dx.plos.org/10.1371/journal.pone.0124473Relations
Is Supplemented By:- [Bioproject]
Title: This is a genome assembly third party annotation of Neospora caninum LIV strain based on strand-specific RNA-sequencing and manual re-annotation identifying novel features of UTRs and non-coding transcripts.Publication Date: 2015-02-26. bioproject: PRJEB7872 Handle: 10754/666436 - [Bioproject]
Title: This is a genome assembly third party annotation of Toxoplasma gondii VEG strain based on strand-specific RNA-sequencing and manual re-annotation identifying novel features of UTRs and non-coding transcripts.Publication Date: 2015-02-26. bioproject: PRJEB7873 Handle: 10754/666442
ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0124473
Scopus Count
Related articles
- Comparative genomics of the apicomplexan parasites Toxoplasma gondii and Neospora caninum: Coccidia differing in host range and transmission strategy.
- Authors: Reid AJ, Vermont SJ, Cotton JA, Harris D, Hill-Cawthorne GA, Könen-Waisman S, Latham SM, Mourier T, Norton R, Quail MA, Sanders M, Shanmugam D, Sohal A, Wasmuth JD, Brunk B, Grigg ME, Howard JC, Parkinson J, Roos DS, Trees AJ, Berriman M, Pain A, Wastling JM
- Issue date: 2012
- Toxoplasma gondii and Neospora caninum induce different host cell responses at proteome-wide phosphorylation events; a step forward for uncovering the biological differences between these closely related parasites.
- Authors: Al-Bajalan MMM, Xia D, Armstrong S, Randle N, Wastling JM
- Issue date: 2017 Oct
- Toxoplasma gondii and Neospora caninum in sparrows (Passer domesticus) in the Northeast of Brazil.
- Authors: Gondim LS, Abe-Sandes K, Uzêda RS, Silva MS, Santos SL, Mota RA, Vilela SM, Gondim LF
- Issue date: 2010 Feb 26
- Molecular assessment of Neospora caninum and Toxoplasma gondii in hooded crows (Corvus cornix) in Tehran, Iran.
- Authors: Abdoli A, Arbabi M, Pirestani M, Mirzaghavami M, Ghaffarifar F, Dalimi A, Sadraei J
- Issue date: 2018 Apr
- ROP18 is a key factor responsible for virulence difference between Toxoplasma gondii and Neospora caninum.
- Authors: Lei T, Wang H, Liu J, Nan H, Liu Q
- Issue date: 2014