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    Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement

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    Type
    Article
    Authors
    He, Li-Sheng
    Xu, Ying
    Matsumura, Kiyotaka
    Zhang, Yu
    Zhang, Gen
    Qi, Shu-Hua
    Qian, Pei-Yuan cc
    KAUST Grant Number
    SA-C0040
    UK-C0016
    Date
    2012-10-24
    Permanent link to this record
    http://hdl.handle.net/10754/550215
    
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    Abstract
    The barnacle Balanus ( = Amphibalanus) amphitrite is a major marine fouling animal. Understanding the molecular mechanism of larval settlement in this species is critical for anti-fouling research. In this study, we cloned one isoform of p38 MAPK (Bar-p38 MAPK) from this species, which shares the significant characteristic of containing a TGY motif with other species such as yeast, Drosophila and humans. The activation of p38 MAPK was detected by an antibody that recognizes the conserved dual phosphorylation sites of TGY. The results showed that phospho-p38 MAPK (pp38 MAPK) was more highly expressed at the cyprid stage, particularly in aged cyprids, in comparison to other stages, including the nauplius and juvenile stages. Immunostaining showed that Bar-p38 MAPK and pp38 MAPK were mainly located at the cyprid antennules, and especially the third and fourth segments, which are responsible for substratum exploration during settlement. The expression and localization patterns of Bar-p38 MAPK suggest its involvement in larval settlement. This postulation was also supported by the larval settlement bioassay with the p38 MAPK inhibitor SB203580. Behavioral analysis by live imaging revealed that the larvae were still capable of exploring the surface of the substratum after SB203580 treatment. This shows that the effect of p38 MAPK on larval settlement might be by regulating the secretion of permanent proteinaceous substances. Furthermore, the level of pp38 MAPK dramatically decreased after full settlement, suggesting that Bar-p38 MAPK maybe plays a role in larval settlement rather than metamorphosis. Finally, we found that Bar-p38 MAPK was highly activated when larvae confronted extracts of adult barnacle containing settlement cues, whereas larvae pre-treated with SB203580 failed to respond to the crude adult extracts.
    Citation
    Evidence for the Involvement of p38 MAPK Activation in Barnacle Larval Settlement 2012, 7 (10):e47195 PLoS ONE
    Sponsors
    This work was supported by an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and grants (N-HKUST602/09 and AoE/P-04/04-II) from the Research Grants Council of the Hong Kong Special Administrative Region to PY Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
    Publisher
    Public Library of Science (PLoS)
    Journal
    PLoS ONE
    DOI
    10.1371/journal.pone.0047195
    PubMed ID
    23115639
    PubMed Central ID
    PMC3480373
    Additional Links
    http://dx.plos.org/10.1371/journal.pone.0047195
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0047195
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