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dc.contributor.authorMarondedze, Claudius
dc.contributor.authorWong, Aloysius Tze
dc.contributor.authorGroen, Arnoud
dc.contributor.authorSerano, Natalia Lorena Gorron
dc.contributor.authorJankovic, Boris R.
dc.contributor.authorLilley, Kathryn
dc.contributor.authorGehring, Christoph A
dc.contributor.authorThomas, Ludivine
dc.date.accessioned2015-04-15T07:22:57Z
dc.date.available2015-04-15T07:22:57Z
dc.date.issued2014-12-31
dc.identifier.citationExploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage 2014, 16 (1):857 International Journal of Molecular Sciences
dc.identifier.issn1422-0067
dc.identifier.pmid25561235
dc.identifier.doi10.3390/ijms16010857
dc.identifier.urihttp://hdl.handle.net/10754/550137
dc.description.abstractThe study of proteomes provides new insights into stimulus-specific responses of protein synthesis and turnover, and the role of post-translational modifications at the systems level. Due to the diverse chemical nature of proteins and shortcomings in the analytical techniques used in their study, only a partial display of the proteome is achieved in any study, and this holds particularly true for plant proteomes. Here we show that different solubilization and separation methods have profound effects on the resulting proteome. In particular, we observed that the type of detergents employed in the solubilization buffer preferentially enriches proteins in different functional categories. These include proteins with a role in signaling, transport, response to temperature stimuli and metabolism. This data may offer a functional bias on comparative analysis studies. In order to obtain a broader coverage, we propose a two-step solubilization protocol with first a detergent-free buffer and then a second step utilizing a combination of two detergents to solubilize proteins.
dc.publisherMDPI AG
dc.relation.urlhttp://www.mdpi.com/1422-0067/16/1/857/
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
dc.subjecttwo-dimensional gel electrophoresis (2DE)
dc.subjectArabidopsis thaliana
dc.subjectdetergent
dc.subjectmass spectrometry (MS)
dc.subjectOFFGEL fractionation
dc.titleExploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalInternational Journal of Molecular Sciences
dc.identifier.pmcidPMC4307279
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCambridge Centre for Proteomics, Cambridge System Biology, University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QR, UK
kaust.personMarondedze, Claudius
kaust.personWong, Aloysius Tze
kaust.personGehring, Christoph A
kaust.personThomas, Ludivine
kaust.personSerano, Natalia Lorena Gorron
kaust.personJankovic, Boris R.
refterms.dateFOA2018-06-14T03:48:49Z


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