Exploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage

Abstract
The study of proteomes provides new insights into stimulus-specific responses of protein synthesis and turnover, and the role of post-translational modifications at the systems level. Due to the diverse chemical nature of proteins and shortcomings in the analytical techniques used in their study, only a partial display of the proteome is achieved in any study, and this holds particularly true for plant proteomes. Here we show that different solubilization and separation methods have profound effects on the resulting proteome. In particular, we observed that the type of detergents employed in the solubilization buffer preferentially enriches proteins in different functional categories. These include proteins with a role in signaling, transport, response to temperature stimuli and metabolism. This data may offer a functional bias on comparative analysis studies. In order to obtain a broader coverage, we propose a two-step solubilization protocol with first a detergent-free buffer and then a second step utilizing a combination of two detergents to solubilize proteins.

Citation
Exploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage 2014, 16 (1):857 International Journal of Molecular Sciences

Publisher
MDPI AG

Journal
International Journal of Molecular Sciences

DOI
10.3390/ijms16010857

PubMed ID
25561235

PubMed Central ID
PMC4307279

Additional Links
http://www.mdpi.com/1422-0067/16/1/857/

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