Exploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage
Type
ArticleAuthors
Marondedze, Claudius
Wong, Aloysius Tze

Groen, Arnoud
Serano, Natalia Lorena Gorron

Jankovic, Boris R.
Lilley, Kathryn
Gehring, Christoph A

Thomas, Ludivine
KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Core Lab
Bioscience Program
Computational Bioscience Research Center (CBRC)
Molecular Signalling Group
Date
2014-12-31Permanent link to this record
http://hdl.handle.net/10754/550137
Metadata
Show full item recordAbstract
The study of proteomes provides new insights into stimulus-specific responses of protein synthesis and turnover, and the role of post-translational modifications at the systems level. Due to the diverse chemical nature of proteins and shortcomings in the analytical techniques used in their study, only a partial display of the proteome is achieved in any study, and this holds particularly true for plant proteomes. Here we show that different solubilization and separation methods have profound effects on the resulting proteome. In particular, we observed that the type of detergents employed in the solubilization buffer preferentially enriches proteins in different functional categories. These include proteins with a role in signaling, transport, response to temperature stimuli and metabolism. This data may offer a functional bias on comparative analysis studies. In order to obtain a broader coverage, we propose a two-step solubilization protocol with first a detergent-free buffer and then a second step utilizing a combination of two detergents to solubilize proteins.Citation
Exploring the Arabidopsis Proteome: Influence of Protein Solubilization Buffers on Proteome Coverage 2014, 16 (1):857 International Journal of Molecular SciencesPublisher
MDPI AGPubMed ID
25561235PubMed Central ID
PMC4307279Additional Links
http://www.mdpi.com/1422-0067/16/1/857/ae974a485f413a2113503eed53cd6c53
10.3390/ijms16010857
Scopus Count
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