Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Tsao, George Sai-wah
Li Lung, Maria
Ngok-shun Wong, Ricky
Ying-kit Yue, Patrick
KAUST DepartmentBioscience Core Lab
Permanent link to this recordhttp://hdl.handle.net/10754/550074
MetadataShow full item record
AbstractExosomes, a group of secreted extracellular nanovesicles containing genetic materials and signaling molecules, play a critical role in intercellular communication. During tumorigenesis, exosomes have been demonstrated to promote tumor angiogenesis and metastasis while their biological functions in nasopharyngeal carcinoma (NPC) are poorly understood. In this study, we focused on the role of NPC-derived exosomes on angiogenesis. Exosomes derived from the NPC C666-1 cells and immortalized nasopharyngeal epithelial cells (NP69 and NP460) were isolated using ultracentrifugation. The molecular profile and biophysical characteristics of exosomes were verified by Western blotting, sucrose density gradient, and electron microscopy. We showed that the C666-1 exosomes (10 and 20 μg/ml) could significantly increase the tubulogenesis, migration and invasion of human umbilical vein endothelial cells (HUVECs) in a dose-dependent manner. Subsequently, an iTRAQ-based quantitative proteomics was used to identify the differentially expressed proteins in C666-1 exosomes. Among the 640 identified proteins, 51 and 89 proteins were considered as up- and down-regulated (≥ 1.5-fold variations) in C666-1 exosomes compared to the normal counterparts, respectively. As expected, pro-angiogenic proteins including intercellular adhesion molecule-1 (ICAM-1) and CD44 variant isoform 5 (CD44v5) are among the up-regulated proteins, whereas angio-suppressive protein, thrombospondin-1 (TSP-1) was down-regulated in C666-1 exosomes. Further confocal microscopic study and Western blotting clearly demonstrated that the alteration of ICAM-1, and TSP-1 expressions in recipient HUVECs are due to internalization of exosomes. Taken together, these data strongly indicated the critical roles of identified angiogenic proteins in the involvement of exosomes-induced angiogenesis, which could potentially be developed as therapeutic targets in future. This article is protected by copyright. All rights reserved.
CitationChan, Yuk‐kit, Huoming Zhang, Pei Liu, George Sai‐wah Tsao, Maria Li Lung, Nai‐ki Mak, Ngok‐shun Wong, and Ying‐kit Yue. "Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins." International Journal of Cancer (2015).
JournalInternational Journal of Cancer
- Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10.
- Authors: Bao L, You B, Shi S, Shan Y, Zhang Q, Yue H, Zhang J, Zhang W, Shi Y, Liu Y, Wang X, Liu D, You Y
- Issue date: 2018 May
- Clinical and biological significance of HAX-1 overexpression in nasopharyngeal carcinoma.
- Authors: You B, Cao X, Shao X, Ni H, Shi S, Shan Y, Gu Z, You Y
- Issue date: 2016 Mar 15
- Overexpression of FOXM1 is associated with metastases of nasopharyngeal carcinoma.
- Authors: Jiang L, Wang P, Chen H
- Issue date: 2014 Nov
- Proteomic analysis of a nasopharyngeal carcinoma cell line and a nasopharyngeal epithelial cell line.
- Authors: Huang PY, Zeng TT, Li MQ, Ban X, Zhu YH, Zhang BZ, Mai HQ, Zhang L, Guan XY, Li Y
- Issue date: 2015 Nov-Dec
- Anti-angiogenic pathway associations of the 3p21.3 mapped BLU gene in nasopharyngeal carcinoma.
- Authors: Cheng Y, Ho RL, Chan KC, Kan R, Tung E, Lung HL, Yau WL, Cheung AK, Ko JM, Zhang ZF, Luo DZ, Feng ZB, Chen S, Guan XY, Kwong D, Stanbridge EJ, Lung ML
- Issue date: 2015 Aug 6