Show simple item record

dc.contributor.authorLi, Yongxin
dc.contributor.authorLi, Zhongrui
dc.contributor.authorYamanaka, Kazuya
dc.contributor.authorXu, Ying
dc.contributor.authorZhang, Weipeng
dc.contributor.authorVlamakis, Hera
dc.contributor.authorKolter, Roberto
dc.contributor.authorMoore, Bradley S.
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2015-03-31T06:17:24Z
dc.date.available2015-03-31T06:17:24Z
dc.date.issued2015-03-24
dc.identifier.citationDirected natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis 2015, 5:9383 Scientific Reports
dc.identifier.issn2045-2322
dc.identifier.pmid25807046
dc.identifier.doi10.1038/srep09383
dc.identifier.urihttp://hdl.handle.net/10754/347333
dc.description.abstractBacilli are ubiquitous low G+C environmental Gram-positive bacteria that produce a wide assortment of specialized small molecules. Although their natural product biosynthetic potential is high, robust molecular tools to support the heterologous expression of large biosynthetic gene clusters in Bacillus hosts are rare. Herein we adapt transformation-associated recombination (TAR) in yeast to design a single genomic capture and expression vector for antibiotic production in Bacillus subtilis. After validating this direct cloning plug-and-playa approach with surfactin, we genetically interrogated amicoumacin biosynthetic gene cluster from the marine isolate Bacillus subtilis 1779. Its heterologous expression allowed us to explore an unusual maturation process involving the N-acyl-asparagine pro-drug intermediates preamicoumacins, which are hydrolyzed by the asparagine-specific peptidase into the active component amicoumacin A. This work represents the first direct cloning based heterologous expression of natural products in the model organism B. subtilis and paves the way to the development of future genome mining efforts in this genus.
dc.description.sponsorshipThis study was generously supported by a grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association, award SA-C0040/UK-C0016 from the King Abdullah University of Science and Technology to P.Y.Q., and funding from the NIH (R01-GM085770) to B.S.M.
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/srep09383
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.titleDirected natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis
dc.typeArticle
dc.identifier.journalScientific Reports
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionSkaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92093, United States
dc.contributor.institutionDepartment of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, United States
dc.contributor.institutionJNC Corporation, Yokohama Research Center, 5-1 Okawa, Kanazawa-ku, Yokohama, Kanagawa 2368605, Japan
dc.contributor.institutionCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, CA 92093, United States
dc.contributor.institutionDivision of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personLi, Yongxin
kaust.personLi, Zhongrui
kaust.personXu, Ying
kaust.personZhang, Weipeng
kaust.personQian, Pei-Yuan
kaust.grant.numberSA-C0040
kaust.grant.numberUK-C0016
kaust.grant.programKAUST Global Collaborative Research Program
refterms.dateFOA2018-06-14T04:59:08Z
dc.date.published-online2015-03-24
dc.date.published-print2015-08


Files in this item

Thumbnail
Name:
srep09383.pdf
Size:
943.9Kb
Format:
PDF
Description:
Main article
Thumbnail
Name:
srep09383-s1.doc
Size:
7.485Mb
Format:
Microsoft Word
Description:
Supplemental files

This item appears in the following Collection(s)

Show simple item record