Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea
KAUST DepartmentRed Sea Research Center (RSRC)
Water Desalination and Reuse Research Center (WDRC)
Environmental Science and Engineering Program
Permanent link to this recordhttp://hdl.handle.net/10754/346877
MetadataShow full item record
AbstractMolecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of Red Sea. Nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. Microbial source tracking revealed the presence of >1 human-associated Bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. Water samples collected from the beaches had occasional exceedances in enterococci numbers, higher total organic carbon (TOC, 1.48-2.18 mg/L) and nitrogen (TN, 0.15-0.27 mg/L) than that detected in the near-shore waters. Enterococci abundances obtained from next-generation sequencing did not correlate well with the cultured enterococci numbers. The abundance of certain genera, for example Arcobacter, Pseudomonas and unclassified Campylobacterales, was observed to exhibit slight correlation with TOC and TN. Low abundance of functional genes accounting for up to 41 copies/L of each Pseudomonas aeruginosa and Campylobacter coli were detected. Arcobacter butzleri was also detected in abundance ranging from 111 to 238 copies/L. Operational taxonomic units (OTUs) associated with cyanobacteria, Prochlorococcus, Ostreococcus spp. and Gramella were more prevalent in waters that were likely impacted by urban runoffs and recreational activities. These OTUs could potentially serve as quantifiable markers indicative of the water quality.
CitationMolecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea 2015, 5:9001 Scientific Reports
PublisherNature Publishing Group
PubMed Central IDPMC4355682
- Specificity and transcriptional activity of microbiota associated with low and high microbial abundance sponges from the Red Sea.
- Authors: Moitinho-Silva L, Bayer K, Cannistraci CV, Giles EC, Ryu T, Seridi L, Ravasi T, Hentschel U
- Issue date: 2014 Mar
- Presence of pathogens and indicator microbes at a non-point source subtropical recreational marine beach.
- Authors: Abdelzaher AM, Wright ME, Ortega C, Solo-Gabriele HM, Miller G, Elmir S, Newman X, Shih P, Bonilla JA, Bonilla TD, Palmer CJ, Scott T, Lukasik J, Harwood VJ, McQuaig S, Sinigalliano C, Gidley M, Plano LR, Zhu X, Wang JD, Fleming LE
- Issue date: 2010 Feb
- Association of fecal indicator bacteria with human viruses and microbial source tracking markers at coastal beaches impacted by nonpoint source pollution.
- Authors: McQuaig S, Griffith J, Harwood VJ
- Issue date: 2012 Sep
- Distribution of potentially pathogenic bacteria as free living and plankton associated in a marine coastal zone.
- Authors: Maugeri TL, Carbone M, Fera MT, Irrera GP, Gugliandolo C
- Issue date: 2004
- Temporal stability of the microbial community in sewage-polluted seawater exposed to natural sunlight cycles and marine microbiota.
- Authors: Sassoubre LM, Yamahara KM, Boehm AB
- Issue date: 2015 Mar