Molecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Environmental Science and Engineering Program
Marine Science Program
Red Sea Research Center (RSRC)
Water Desalination and Reuse Research Center (WDRC)
Online Publication Date2015-03-11
Print Publication Date2015-08
Permanent link to this recordhttp://hdl.handle.net/10754/346877
MetadataShow full item record
AbstractMolecular-based approaches were used to characterize the coastal microbiota and to elucidate the trophic state of Red Sea. Nutrient content and enterococci numbers were monitored, and used to correlate with the abundance of microbial markers. Microbial source tracking revealed the presence of >1 human-associated Bacteroides spp. at some of the near-shore sampling sites and at a heavily frequented beach. Water samples collected from the beaches had occasional exceedances in enterococci numbers, higher total organic carbon (TOC, 1.48-2.18 mg/L) and nitrogen (TN, 0.15-0.27 mg/L) than that detected in the near-shore waters. Enterococci abundances obtained from next-generation sequencing did not correlate well with the cultured enterococci numbers. The abundance of certain genera, for example Arcobacter, Pseudomonas and unclassified Campylobacterales, was observed to exhibit slight correlation with TOC and TN. Low abundance of functional genes accounting for up to 41 copies/L of each Pseudomonas aeruginosa and Campylobacter coli were detected. Arcobacter butzleri was also detected in abundance ranging from 111 to 238 copies/L. Operational taxonomic units (OTUs) associated with cyanobacteria, Prochlorococcus, Ostreococcus spp. and Gramella were more prevalent in waters that were likely impacted by urban runoffs and recreational activities. These OTUs could potentially serve as quantifiable markers indicative of the water quality.
CitationMolecular-based approaches to characterize coastal microbial community and their potential relation to the trophic state of Red Sea 2015, 5:9001 Scientific Reports
PubMed Central IDPMC4355682
CollectionsArticles; Biological and Environmental Science and Engineering (BESE) Division; Red Sea Research Center (RSRC); Environmental Science and Engineering Program; Marine Science Program; Water Desalination and Reuse Research Center (WDRC); Plankton Genomics, part of the Global Ocean Genome Project
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