Arabidopsis flower specific defense gene expression patterns affect resistance to pathogens
Dawe, Adam Sean
Gehring, Christoph A
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Molecular Signalling Group
Office of the VP
Plant Stress Genomics Research Lab
Permanent link to this recordhttp://hdl.handle.net/10754/346612
MetadataShow full item record
AbstractWe investigated whether the Arabidopsis flower evolved protective measures to increase reproductive success. Firstly, analyses of available transcriptome data show that the most highly expressed transcripts in the closed sepal (stage 12) are enriched in genes with roles in responses to chemical stimuli and cellular metabolic processes. At stage 15, there is enrichment in transcripts with a role in responses to biotic stimuli. Comparative analyses between the sepal and petal in the open flower mark an over-representation of transcripts with a role in responses to stress and catalytic activity. Secondly, the content of the biotic defense-associated phytohormone salicylic acid (SA) in sepals and petals is significantly higher than in leaves. To understand whether the high levels of stress responsive transcripts and the higher SA content affect defense, wild-type plants (Col-0) and transgenic plants defective in SA accumulation (nahG) were challenged with the biotrophic fungus Golovinomyces cichoracearum, the causal agent of powdery mildew, and the necrotrophic fungus Botrytis cinerea. NahG leaves were more sensitive than those of Col-0, suggesting that in leaves SA has a role in the defense against biotrophs. In contrast, sepals and petals of both genotypes were resistant to G. cichoracearum, indicating that in the flower, resistance to the biotrophic pathogen is not critically dependent on SA, but likely dependent on the up-regulation of stress-responsive genes. Since sepals and petals of both genotypes are equally susceptible to B. cinerea, we conclude that neither stress-response genes nor increased SA accumulation offers protection against the necrotrophic pathogen. These results are interpreted in the light of the distinctive role of the flower and we propose that in the early stages, the sepal may act as a chemical defense barrier of the developing reproductive structures against biotrophic pathogens.
CitationArabidopsis flower specific defense gene expression patterns affect resistance to pathogens 2015, 6 Frontiers in Plant Science
PublisherFrontiers Media SA
JournalFrontiers in Plant Science
PubMed Central IDPMC4335275
- Ectopic Expression of the Wild Grape WRKY Transcription Factor VqWRKY52 in <i>Arabidopsis thaliana</i> Enhances Resistance to the Biotrophic Pathogen Powdery Mildew But Not to the Necrotrophic Pathogen <i>Botrytis cinerea</i>.
- Authors: Wang X, Guo R, Tu M, Wang D, Guo C, Wan R, Li Z, Wang X
- Issue date: 2017
- Heterologous Expression of the Grapevine <i>JAZ7</i> Gene in Arabidopsis Confers Enhanced Resistance to Powdery Mildew but Not to <i>Botrytis cinerea</i>.
- Authors: Hanif M, Rahman MU, Gao M, Yang J, Ahmad B, Yan X, Wang X
- Issue date: 2018 Dec 5
- Rhamnolipids elicit defense responses and induce disease resistance against biotrophic, hemibiotrophic, and necrotrophic pathogens that require different signaling pathways in Arabidopsis and highlight a central role for salicylic acid.
- Authors: Sanchez L, Courteaux B, Hubert J, Kauffmann S, Renault JH, Clément C, Baillieul F, Dorey S
- Issue date: 2012 Nov
- The SA-dependent defense pathway is active against different pathogens in tomato and tobacco.
- Authors: Achuo AE, Audenaert K, Meziane H, Höfte M
- Issue date: 2002
- PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana.
- Authors: Duan Y, Jiang Y, Ye S, Karim A, Ling Z, He Y, Yang S, Luo K
- Issue date: 2015 May