Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging
Type
ArticleAuthors
Tirinato, Luca
Liberale, Carlo

Di Franco, Simone
Candeloro, Patrizio

Benfante, Antonina
La Rocca, Rosanna
Potze, Lisette
Marotta, Roberto
Ruffilli, Roberta
Rajamanickam, Vijayakumar Palanisamy
Malerba, Mario
De Angelis, Francesco
Falqui, Andrea

Carbone, Ennio
Todaro, Matilde
Medema, Jan Paul
Stassi, Giorgio
Di Fabrizio, Enzo M.

KAUST Department
Biological and Environmental Sciences and Engineering (BESE) DivisionBioscience Program
Label-Free Optical Microscopy for Biology Lab
Material Science and Engineering Program
Physical Science and Engineering (PSE) Division
Date
2014-12-18Online Publication Date
2014-12-18Print Publication Date
2015-01Permanent link to this record
http://hdl.handle.net/10754/337898
Metadata
Show full item recordAbstract
The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs.Citation
Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging 2015, 33 (1):35 STEM CELLSPublisher
WileyJournal
STEM CELLSPubMed ID
25186497PubMed Central ID
PMC4311668Additional Links
http://doi.wiley.com/10.1002/stem.1837ae974a485f413a2113503eed53cd6c53
10.1002/stem.1837
Scopus Count
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