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    Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

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    lipid droplets.pdf
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    Type
    Article
    Authors
    Tirinato, Luca cc
    Liberale, Carlo cc
    Di Franco, Simone
    Candeloro, Patrizio cc
    Benfante, Antonina
    La Rocca, Rosanna
    Potze, Lisette
    Marotta, Roberto
    Ruffilli, Roberta
    Rajamanickam, Vijayakumar Palanisamy
    Malerba, Mario
    De Angelis, Francesco
    Falqui, Andrea cc
    Carbone, Ennio
    Todaro, Matilde
    Medema, Jan Paul
    Stassi, Giorgio
    Di Fabrizio, Enzo M. cc
    KAUST Department
    Biological and Environmental Sciences and Engineering (BESE) Division
    Bioscience Program
    Label-Free Optical Microscopy for Biology Lab
    Material Science and Engineering Program
    Physical Science and Engineering (PSE) Division
    Date
    2014-12-18
    Online Publication Date
    2014-12-18
    Print Publication Date
    2015-01
    Permanent link to this record
    http://hdl.handle.net/10754/337898
    
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    Abstract
    The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs.
    Citation
    Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging 2015, 33 (1):35 STEM CELLS
    Publisher
    Wiley
    Journal
    STEM CELLS
    DOI
    10.1002/stem.1837
    PubMed ID
    25186497
    PubMed Central ID
    PMC4311668
    Additional Links
    http://doi.wiley.com/10.1002/stem.1837
    ae974a485f413a2113503eed53cd6c53
    10.1002/stem.1837
    Scopus Count
    Collections
    Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Physical Science and Engineering (PSE) Division; Material Science and Engineering Program

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