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dc.contributor.authorLa Rocca, Rosanna
dc.contributor.authorTallerico, Rossana
dc.contributor.authorTalib Hassan, Almosawy
dc.contributor.authorDas, Gobind
dc.contributor.authorTadepally, Lakshmikanth
dc.contributor.authorMatteucci, Marco
dc.contributor.authorLiberale, Carlo
dc.contributor.authorMesuraca, Maria
dc.contributor.authorScumaci, Domenica
dc.contributor.authorGentile, Francesco
dc.contributor.authorCojoc, Gheorghe
dc.contributor.authorPerozziello, Gerardo
dc.contributor.authorAmmendolia, Antonio
dc.contributor.authorGallo, Adriana
dc.contributor.authorKärre, Klas
dc.contributor.authorCuda, Giovanni
dc.contributor.authorCandeloro, Patrizio
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.authorCarbone, Ennio
dc.date.accessioned2015-01-04T13:48:11Z
dc.date.available2015-01-04T13:48:11Z
dc.date.issued2014-12-26
dc.identifier.citationLa Rocca R, Tallerico R, Talib Hassan A, Das G, Tadepally L, et al. (2014) Mechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane. PLoS ONE 9(12): e111758. doi:10.1371/journal.pone.0111758
dc.identifier.issn1932-6203
dc.identifier.pmid25541692
dc.identifier.doi10.1371/journal.pone.0111758
dc.identifier.urihttp://hdl.handle.net/10754/337778
dc.description.abstractIn our body, cells are continuously exposed to physical forces that can regulate different cell functions such as cell proliferation, differentiation and death. In this work, we employed two different strategies to mechanically stress cancer cells. The cancer and healthy cell populations were treated either with mechanical stress delivered by a micropump (fabricated by deep X-ray nanolithography) or by ultrasound wave stimuli. A specific down-regulation of Major Histocompatibility Complex (MHC) class I molecules expression on cancer cell membrane compared to different kinds of healthy cells (fibroblasts, macrophages, dendritic and lymphocyte cells) was observed, stimulating the cells with forces in the range of nano-newton, and pressures between 1 and 10 bar (1 bar = 100.000 Pascal), depending on the devices used. Moreover, Raman spectroscopy analysis, after mechanical treatment, in the range between 700–1800 cm−1, indicated a relative concentration variation of MHC class I. PCA analysis was also performed to distinguish control and stressed cells within different cell lines. These mechanical induced phenotypic changes increase the tumor immunogenicity, as revealed by the related increased susceptibility to Natural Killer (NK) cells cytotoxic recognition.
dc.description.sponsorshipEnnio Carbone's work has been supported by a UICC International Cancer Technology Transfer Fellowship, grant AIRC-IG 10189, and grant AIRC 15521. Rossana Tallerico is a Post Doc awarded by triennial fellowships “Luciana Selce” FIRC. Giovanni Cuda has been supported by PON01_02834 Prometeo (Ministry of Education and Research) and PONa3_00435 Biomedpark@UMG (Ministry of Education and Research). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0111758
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectCancer treatment
dc.subjectCell membranes
dc.subjectLymphocytes
dc.subjectMHC class I genes
dc.subjectMajor histocompatibility complex
dc.subjectMechanical stress
dc.subjectMembrane proteins
dc.subjectNK cells
dc.titleMechanical Stress Downregulates MHC Class I Expression on Human Cancer Cell Membrane
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentLabel-Free Optical Microscopy for Biology Lab
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC4277281
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University of “Magna Graecia”, Catanzaro, Italy
dc.contributor.institutionItalian Institute of Technology (IIT), Genova, Italy
dc.contributor.institutionDepartment of Microbiology, College of Medicine, University of Thi-Qar, Nasseriah, Iraq
dc.contributor.institutionScience for Life Laboratory, Department of Medicine, Karolinska Institute, Stockholm, Sweden
dc.contributor.institutionNanotech Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens Lyngby, Denmark
dc.contributor.institutionDepartment of Surgical and Medical Sciences, University of “Magna Graecia”, Catanzaro, Italy
dc.contributor.institutionEndocrinology and Experimental Oncology Institute, CNR, Napoli, Italy
dc.contributor.institutionDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personDas, Gobind
kaust.personLiberale, Carlo
kaust.personDi Fabrizio, Enzo M.
refterms.dateFOA2018-06-13T16:08:22Z


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