Standardizing the experimental conditions for using urine in NMR-based metabolomic studies with a particular focus on diagnostic studies: a review
Name:
art10.1007s11306-014-0746-7.pdf
Size:
452.4Kb
Format:
PDF
Description:
Article - Full Text
Type
ArticleAuthors
Emwas, Abdul-Hamid M.Luchinat, Claudio
Turano, Paola
Tenori, Leonardo
Roy, Raja
Salek, Reza M.
Ryan, Danielle
Merzaban, Jasmeen

Kaddurah-Daouk, Rima
Zeri, Ana Carolina
Nagana Gowda, G. A.
Raftery, Daniel
Wang, Yulan
Brennan, Lorraine
Wishart, David S.
KAUST Department
Advanced Nanofabrication, Imaging and Characterization Core LabBiological and Environmental Sciences and Engineering (BESE) Division
Bioscience Program
NMR
Date
2014-11-21Online Publication Date
2014-11-21Print Publication Date
2015-08Permanent link to this record
http://hdl.handle.net/10754/337011
Metadata
Show full item recordAbstract
The metabolic composition of human biofluids can provide important diagnostic and prognostic information. Among the biofluids most commonly analyzed in metabolomic studies, urine appears to be particularly useful. It is abundant, readily available, easily stored and can be collected by simple, noninvasive techniques. Moreover, given its chemical complexity, urine is particularly rich in potential disease biomarkers. This makes it an ideal biofluid for detecting or monitoring disease processes. Among the metabolomic tools available for urine analysis, NMR spectroscopy has proven to be particularly well-suited, because the technique is highly reproducible and requires minimal sample handling. As it permits the identification and quantification of a wide range of compounds, independent of their chemical properties, NMR spectroscopy has been frequently used to detect or discover disease fingerprints and biomarkers in urine. Although protocols for NMR data acquisition and processing have been standardized, no consensus on protocols for urine sample selection, collection, storage and preparation in NMR-based metabolomic studies have been developed. This lack of consensus may be leading to spurious biomarkers being reported and may account for a general lack of reproducibility between laboratories. Here, we review a large number of published studies on NMR-based urine metabolic profiling with the aim of identifying key variables that may affect the results of metabolomics studies. From this survey, we identify a number of issues that require either standardization or careful accounting in experimental design and provide some recommendations for urine collection, sample preparation and data acquisition.Citation
Standardizing the experimental conditions for using urine in NMR-based metabolomic studies with a particular focus on diagnostic studies: a review 2014 MetabolomicsPublisher
Springer NatureJournal
MetabolomicsPubMed ID
26109927PubMed Central ID
PMC4475544Additional Links
http://link.springer.com/10.1007/s11306-014-0746-7ae974a485f413a2113503eed53cd6c53
10.1007/s11306-014-0746-7
Scopus Count
The following license files are associated with this item:
Related articles
- Recommendations and Standardization of Biomarker Quantification Using NMR-Based Metabolomics with Particular Focus on Urinary Analysis.
- Authors: Emwas AH, Roy R, McKay RT, Ryan D, Brennan L, Tenori L, Luchinat C, Gao X, Zeri AC, Gowda GA, Raftery D, Steinbeck C, Salek RM, Wishart DS
- Issue date: 2016 Feb 5
- (1)H NMR-based metabolite profiling workflow to reduce inter-sample chemical shift variations in urine samples for improved biomarker discovery.
- Authors: Gil RB, Lehmann R, Schmitt-Kopplin P, Heinzmann SS
- Issue date: 2016 Jul
- Metabolomics: the "new clinical chemistry" for personalized neonatal medicine.
- Authors: Antonucci R, Atzori L, Barberini L, Fanos V
- Issue date: 2010 Jun
- Nuclear magnetic resonance metabolomic profiling of urine provides a noninvasive alternative to the identification of biomarkers associated with endometriosis.
- Authors: Vicente-Muñoz S, Morcillo I, Puchades-Carrasco L, Payá V, Pellicer A, Pineda-Lucena A
- Issue date: 2015 Nov
- Biofluid metabonomics using (1)H NMR spectroscopy: the road to biomarker discovery in gastroenterology and hepatology.
- Authors: Patel NR, McPhail MJ, Shariff MI, Keun HC, Taylor-Robinson SD
- Issue date: 2012 Apr