Standardizing the experimental conditions for using urine in NMR-based metabolomic studies with a particular focus on diagnostic studies: a review
AuthorsEmwas, Abdul-Hamid M.
Salek, Reza M.
Zeri, Ana Carolina
Nagana Gowda, G. A.
Wishart, David S.
KAUST DepartmentAdvanced Nanofabrication, Imaging and Characterization Core Lab
Biological and Environmental Sciences and Engineering (BESE) Division
Online Publication Date2014-11-21
Print Publication Date2015-08
Permanent link to this recordhttp://hdl.handle.net/10754/337011
MetadataShow full item record
AbstractThe metabolic composition of human biofluids can provide important diagnostic and prognostic information. Among the biofluids most commonly analyzed in metabolomic studies, urine appears to be particularly useful. It is abundant, readily available, easily stored and can be collected by simple, noninvasive techniques. Moreover, given its chemical complexity, urine is particularly rich in potential disease biomarkers. This makes it an ideal biofluid for detecting or monitoring disease processes. Among the metabolomic tools available for urine analysis, NMR spectroscopy has proven to be particularly well-suited, because the technique is highly reproducible and requires minimal sample handling. As it permits the identification and quantification of a wide range of compounds, independent of their chemical properties, NMR spectroscopy has been frequently used to detect or discover disease fingerprints and biomarkers in urine. Although protocols for NMR data acquisition and processing have been standardized, no consensus on protocols for urine sample selection, collection, storage and preparation in NMR-based metabolomic studies have been developed. This lack of consensus may be leading to spurious biomarkers being reported and may account for a general lack of reproducibility between laboratories. Here, we review a large number of published studies on NMR-based urine metabolic profiling with the aim of identifying key variables that may affect the results of metabolomics studies. From this survey, we identify a number of issues that require either standardization or careful accounting in experimental design and provide some recommendations for urine collection, sample preparation and data acquisition.
CitationStandardizing the experimental conditions for using urine in NMR-based metabolomic studies with a particular focus on diagnostic studies: a review 2014 Metabolomics
PubMed Central IDPMC4475544
The following license files are associated with this item:
- Recommendations and Standardization of Biomarker Quantification Using NMR-Based Metabolomics with Particular Focus on Urinary Analysis.
- Authors: Emwas AH, Roy R, McKay RT, Ryan D, Brennan L, Tenori L, Luchinat C, Gao X, Zeri AC, Gowda GA, Raftery D, Steinbeck C, Salek RM, Wishart DS
- Issue date: 2016 Feb 5
- (1)H NMR-based metabolite profiling workflow to reduce inter-sample chemical shift variations in urine samples for improved biomarker discovery.
- Authors: Gil RB, Lehmann R, Schmitt-Kopplin P, Heinzmann SS
- Issue date: 2016 Jul
- Metabolomics: the "new clinical chemistry" for personalized neonatal medicine.
- Authors: Antonucci R, Atzori L, Barberini L, Fanos V
- Issue date: 2010 Jun
- Precision high-throughput proton NMR spectroscopy of human urine, serum, and plasma for large-scale metabolic phenotyping.
- Authors: Dona AC, Jiménez B, Schäfer H, Humpfer E, Spraul M, Lewis MR, Pearce JT, Holmes E, Lindon JC, Nicholson JK
- Issue date: 2014 Oct 7
- Nuclear magnetic resonance metabolomic profiling of urine provides a noninvasive alternative to the identification of biomarkers associated with endometriosis.
- Authors: Vicente-Muñoz S, Morcillo I, Puchades-Carrasco L, Payá V, Pellicer A, Pineda-Lucena A
- Issue date: 2015 Nov