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dc.contributor.authorTurek, Ilona
dc.contributor.authorMarondedze, Claudius
dc.contributor.authorWheeler, Janet I.
dc.contributor.authorGehring, Christoph A
dc.contributor.authorIrving, Helen R.
dc.date.accessioned2014-12-01T15:45:30Z
dc.date.available2014-12-01T15:45:30Z
dc.date.issued2014-11-26
dc.identifier.citationTurek I, Marondedze C, Wheeler JI, Gehring C and Irving HR (2014) Plant natriuretic peptides induce proteins diagnostic for an adaptive response to stress. Front. Plant Sci. 5:661. doi: 10.3389/fpls.2014.00661
dc.identifier.issn1664-462X
dc.identifier.pmid25505478
dc.identifier.doi10.3389/fpls.2014.00661
dc.identifier.urihttp://hdl.handle.net/10754/336429
dc.description.abstractIn plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into the apoplast, are systemically mobile and elicit a range of responses signaling via cGMP. The PNP-dependent responses include tissue specific modifications of cation transport and changes in stomatal conductance and the photosynthetic rate. PNP also has a critical role in host defense responses. Surprisingly, PNP-homologs are produced by several plant pathogens during host colonization suppressing host defense responses. Here we show that a synthetic peptide representing the biologically active fragment of the Arabidopsis thaliana PNP (AtPNP-A) induces the production of reactive oxygen species in suspension-cultured A. thaliana (Col-0) cells. To identify proteins whose expression changes in an AtPNP-A dependent manner, we undertook a quantitative proteomic approach, employing tandem mass tag (TMT) labeling, to reveal temporal responses of suspension-cultured cells to 1 nM and 10 pM PNP at two different time-points post-treatment. Both concentrations yield a distinct differential proteome signature. Since only the higher (1 nM) concentration induces a ROS response, we conclude that the proteome response at the lower concentration reflects a ROS independent response. Furthermore, treatment with 1 nM PNP results in an over-representation of the gene ontology (GO) terms “oxidation-reduction process,” “translation” and “response to salt stress” and this is consistent with a role of AtPNP-A in the adaptation to environmental stress conditions.
dc.language.isoen
dc.publisherFrontiers Media SA
dc.relation.urlhttp://www.frontiersin.org/Plant_Proteomics/10.3389/fpls.2014.00661/abstract
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.subjectplant natriuretic peptide
dc.subjectpeptide hormone signaling
dc.subjectplant homeostasis
dc.subjectmolecular mimicry
dc.subjectsalt stress
dc.subjectreactive oxygen species
dc.titlePlant natriuretic peptides induce proteins diagnostic for an adaptive response to stress
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalFrontiers in Plant Science
dc.identifier.pmcidPMC4244590
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDrug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, VIC, Australia
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personMarondedze, Claudius
kaust.personGehring, Christoph A.
kaust.personTurek, Lions
refterms.dateFOA2018-06-14T03:48:43Z


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