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dc.contributor.authorCesca, F.
dc.contributor.authorLimongi, Tania
dc.contributor.authorAccardo, A.
dc.contributor.authorRocchi, A.
dc.contributor.authorOrlando, M.
dc.contributor.authorShalabaeva, V.
dc.contributor.authorDi Fabrizio, Enzo M.
dc.contributor.authorBenfenati, F.
dc.date.accessioned2014-11-25T13:40:38Z
dc.date.available2014-11-25T13:40:38Z
dc.date.issued2014
dc.identifier.citationCesca, F., Limongi, T., Accardo, A., Rocchi, A., Orlando, M., Shalabaeva, V., . . . Benfenati, F. (2014). Fabrication of biocompatible free-standing nanopatterned films for primary neuronal cultures. RSC Advances, 4(86), 45696-45702. doi: 10.1039/C4RA08361J
dc.identifier.issn2046-2069
dc.identifier.doi10.1039/C4RA08361J
dc.identifier.urihttp://hdl.handle.net/10754/336067
dc.description.abstractDevising and constructing biocompatible devices for nervous system regeneration is an extremely challenging task. Besides tackling the issue of biocompatibility, biomaterials for neuroscience applications should mimic the complex environment of the extracellular matrix, which in vivo provides neurons with a series of cues and signals to guide cells towards their appropriate targets. In this work, a novel nanopatterned biocompatible poly-ε-caprolactone (PCL) film is realized to assist the attachment and growth of primary hippocampal neurons. Costly and time-consuming processes can be avoided using plasma-surface nanotexturing obtained by a mixed gas SF6/Ar at −5 °C. The intrinsic composition and line topography of nanopatterned PCL ensure healthy development of the neuronal network, as shown by confocal microscopy, by analysing the expression of a range of neuronal markers typical of mature cultures, as well as by scanning electron microscopy. In addition, we show that surface nanopatterning improves differentiation of neurons compared to flat PCL films, while no neural growth was observed on either flat or nanopatterned substrates in the absence of a poly-D-lysine coating. Thus, we successfully optimized a nanofabrication protocol to obtain nanostructured PCL layers endowed with several mechanical and structural characteristics that make them a promising, versatile tool for future tissue engineering studies aimed at neural tissue regeneration.
dc.language.isoen
dc.publisherRoyal Society of Chemistry (RSC)
dc.relation.urlhttp://xlink.rsc.org/?DOI=C4RA08361J
dc.rightsThis article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
dc.titleFabrication of biocompatible free-standing nanopatterned films for primary neuronal cultures
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMaterial Science and Engineering Program
dc.contributor.departmentPhysical Science and Engineering (PSE) Division
dc.identifier.journalRSC Adv.
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionNeuroscience and Brain Technologies Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
dc.contributor.institutionNanostructures Department, Istituto Italiano di Tecnologia, Via Morego 30, 16163 Genova, Italy
dc.contributor.institutionBIONEM, Bio-Nanotechnology and Engineering for Medicine, Department of Experimental and Clinical Medicine, University of Magna Graecia Viale Europa, Germaneto, 88100 Catanzaro, Italy
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personLimongi, Tania
kaust.personDi Fabrizio, Enzo M.
refterms.dateFOA2018-06-13T16:03:34Z


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