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dc.contributor.authorTehseen, Muhammad
dc.contributor.authorLiao, Chunyan
dc.contributor.authorDacres, Helen
dc.contributor.authorDumancic, Mira
dc.contributor.authorTrowell, Stephen
dc.contributor.authorAnderson, Alisha
dc.date.accessioned2014-11-16T11:38:57Z
dc.date.available2014-11-16T11:38:57Z
dc.date.issued2014-09-25
dc.identifier.citationOligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast 2014, 9 (9):e108680 PLoS ONE
dc.identifier.issn1932-6203
dc.identifier.pmid25254556
dc.identifier.doi10.1371/journal.pone.0108680
dc.identifier.urihttp://hdl.handle.net/10754/334973
dc.description.abstractIt is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
dc.description.sponsorshipThe research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0108680
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectcell fusion
dc.subjectcell membranes
dc.subjectco-immunoprecipitation
dc.subjectnematoda
dc.subjectOlfactory receptor neurons
dc.subjectpolymerase chain reaction
dc.subjectTranscription Factors
dc.subjectYeast two-hybrid assays
dc.titleOligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC4177895
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionCSIRO Food Futures National Research Flagship & CSIRO Ecosystem Sciences, Australia, Canberra, ACT, Australia
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personTehseen, Muhammad
refterms.dateFOA2018-06-13T16:04:45Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.