Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast
KAUST DepartmentDesert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/334973
MetadataShow full item record
AbstractIt is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.
CitationOligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast 2014, 9 (9):e108680 PLoS ONE
SponsorsThe research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC4177895
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Piezoelectric biosensor using olfactory receptor protein expressed in Escherichia coli.
- Authors: Sung JH, Ko HJ, Park TH
- Issue date: 2006 Apr 15
- An ER complex of ODR-4 and ODR-8/Ufm1 specific protease 2 promotes GPCR maturation by a Ufm1-independent mechanism.
- Authors: Chen C, Itakura E, Weber KP, Hegde RS, de Bono M
- Issue date: 2014 Mar
- Screening of odor-receptor pairs in Caenorhabditis elegans reveals different receptors for high and low odor concentrations.
- Authors: Taniguchi G, Uozumi T, Kiriyama K, Kamizaki T, Hirotsu T
- Issue date: 2014 Apr 29
- Greatly enhanced detection of a volatile ligand at femtomolar levels using bioluminescence resonance energy transfer (BRET).
- Authors: Dacres H, Wang J, Leitch V, Horne I, Anderson AR, Trowell SC
- Issue date: 2011 Nov 15
- Deciphering activation of olfactory receptors using heterologous expression in Saccharomyces cerevisiae and bioluminescence resonance energy transfer.
- Authors: Sanz G, Pajot-Augy E
- Issue date: 2013