Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast
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ArticleKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionCenter for Desert Agriculture
Date
2014-09-25Permanent link to this record
http://hdl.handle.net/10754/334973
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It is widely accepted that vertebrate G-Protein Coupled Receptors (GPCRs) associate with each other as homo- or hetero-dimers or higher-order oligomers. The C. elegans genome encodes hundreds of olfactory GPCRs, which may be expressed in fewer than a dozen chemosensory neurons, suggesting an opportunity for oligomerisation. Here we show, using three independent lines of evidence: co-immunoprecipitation, bioluminescence resonance energy transfer and a yeast two-hybrid assay that nematode olfactory receptors (ORs) oligomerise when heterologously expressed in yeast. Specifically, the nematode receptor ODR-10 is able to homo-oligomerise and can also form heteromers with the related nematode receptor STR-112. ODR-10 also oligomerised with the rat I7 OR but did not oligomerise with the human somatostatin receptor 5, a neuropeptide receptor. In this study, the question of functional relevance was not addressed and remains to be investigated.Citation
Oligomerisation of C. elegans Olfactory Receptors, ODR-10 and STR-112, in Yeast 2014, 9 (9):e108680 PLoS ONESponsors
The research is funded by CSIRO. However, the funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Publisher
Public Library of Science (PLoS)Journal
PLoS ONEPubMed ID
25254556PubMed Central ID
PMC4177895Additional Links
http://dx.plos.org/10.1371/journal.pone.0108680ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0108680
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