Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.)
AuthorsBanguera Hinestroza, Eulalia
Saenz Agudelo, Pablo
Berumen, Michael L.
Voolstra, Christian R.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Marine Science Program
Red Sea Research Center (RSRC)
Reef Ecology Lab
Reef Genomics Lab
Online Publication Date2013-01-09
Print Publication Date2013-06
Permanent link to this recordhttp://hdl.handle.net/10754/334649
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AbstractA total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified successfully and tested in 24 individuals from samples belonging to a single population from the central region of the Red Sea. The number of alleles ranged from 3 to 15 alleles per locus, while observed heterozygosity ranged from 0. 292 to 0. 95. Six of these loci showed significant deviations from Hardy-Weinberg equilibrium (HWE) expectations, and 4/136 paired loci comparisons suggested linkage disequilibrium after Bonferroni corrections. After excluding loci with significant HWE deviation and evidence of null alleles, average genetic diversity over loci in the population studied (N = 24, Nloci = 11) was 0. 701 ± 0. 380. This indicates that these loci can be used effectively to evaluate genetic diversity and undertake population genetics studies in Stylophora sp. populations. 2013 The Author(s).
CitationBanguera-Hinestroza E, Saenz-Agudelo P, Bayer T, Berumen ML, Voolstra CR (2013) Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.). Conservation Genet Resour 5: 561-563. doi:10.1007/s12686-012-9852-x.
JournalConservation Genetics Resources
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