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dc.contributor.authorBelfield, E.J.
dc.contributor.authorGan, X.
dc.contributor.authorMithani, A.
dc.contributor.authorBrown, C.
dc.contributor.authorJiang, C.
dc.contributor.authorFranklin, K.
dc.contributor.authorAlvey, E.
dc.contributor.authorWibowo, Anjar Tri
dc.contributor.authorJung, M.
dc.contributor.authorBailey, K.
dc.contributor.authorKalwani, Sharan
dc.contributor.authorRagoussis, J.
dc.contributor.authorMott, R.
dc.contributor.authorHarberd, N.P.
dc.date.accessioned2014-11-11T14:32:58Z
dc.date.available2014-11-11T14:32:58Z
dc.date.issued2012-04-12
dc.identifier.citationBelfield EJ, Gan X, Mithani A, Brown C, Jiang C, et al. (2012) Genome-wide analysis of mutations in mutant lineages selected following fast-neutron irradiation mutagenesis of Arabidopsis thaliana. Genome Research 22: 1306-1315. doi:10.1101/gr.131474.111.
dc.identifier.issn10889051
dc.identifier.pmid22499668
dc.identifier.doi10.1101/gr.131474.111
dc.identifier.urihttp://hdl.handle.net/10754/334634
dc.description.abstractIonizing radiation has long been known to induce heritable mutagenic change in DNA sequence. However, the genome-wide effect of radiation is not well understood. Here we report the molecular properties and frequency of mutations in phenotypically selected mutant lines isolated following exposure of the genetic model flowering plant Arabidopsis thaliana to fast neutrons (FNs). Previous studies suggested that FNs predominantly induce deletions longer than a kilobase in A. thaliana. However, we found a higher frequency of single base substitution than deletion mutations. While the overall frequency and molecular spectrum of fast-neutron (FN)-induced single base substitutions differed substantially from those of "background" mutations arising spontaneously in laboratory-grown plants, G:C>A:T transitions were favored in both. We found that FN-induced G:C>A:T transitions were concentrated at pyrimidine dinucleotide sites, suggesting that FNs promote the formation of mutational covalent linkages between adjacent pyrimidine residues. In addition, we found that FNs induced more single base than large deletions, and that these single base deletions were possibly caused by replication slippage. Our observations provide an initial picture of the genome-wide molecular profile of mutations induced in A. thaliana by FN irradiation and are particularly informative of the nature and extent of genome-wide mutation in lines selected on the basis of mutant phenotypes from FN-mutagenized A. thaliana populations.
dc.language.isoen
dc.publisherCold Spring Harbor Laboratory Press
dc.rightsThis article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.
dc.rightsArchived with thanks to Genome Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/
dc.subjectpyrimidine
dc.subjectArabidopsis
dc.subjectfast neutron therapy
dc.subjectgene deletion
dc.subjectgene frequency
dc.subjectgenetic association
dc.subjectgenetic model
dc.subjectindel mutation
dc.subjectphenotype
dc.subjectplant genome
dc.subjectradiation mutagenesis
dc.subjectChromosome Aberrations
dc.subjectChromosomes, Plant
dc.subjectDNA Mutational Analysis
dc.subjectDNA Replication
dc.subjectDNA, Plant
dc.subjectFast Neutrons
dc.subjectGenome, Plant
dc.subjectINDEL Mutation
dc.subjectPhenotype
dc.subjectPoint Mutation
dc.subjectPyrimidine Nucleotides
dc.subjectSequence Deletion
dc.subjectArabidopsis thaliana
dc.subjectMagnoliophyta
dc.titleGenome-wide analysis of mutations in mutant lineages selected following fast-neutron irradiation mutagenesis of Arabidopsis thaliana
dc.typeArticle
dc.contributor.departmentBioscience Program
dc.contributor.departmentInformation Technology Department
dc.contributor.departmentOffice of the CIO
dc.contributor.departmentPlant Stress Genomics Research Lab
dc.contributor.departmentResearch Computing
dc.identifier.journalGenome Research
dc.identifier.pmcidPMC3396371
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom
dc.contributor.institutionWellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, United Kingdom
dc.contributor.institutionSchool of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom
dc.contributor.institutionDepartment of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom
dc.contributor.institutionOxford e-Research Centre, University of Oxford, Oxford OX1 3QG, United Kingdom
dc.contributor.institutionDepartment of Biology, LUMS School of Science and Engineering, Sector U-DHA, Lahore 54792, Pakistan
dc.contributor.institutionDepartment of Life Sciences, University of Warwick, Wellesbourne, Warwick CV35 9EF, United Kingdom
dc.contributor.institution1142 Terra Ct., Rochester, MI 48306, United States
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personWibowo, Anjar Tri
kaust.personKalwani, Sharan
refterms.dateFOA2018-06-14T07:18:25Z
dc.date.published-online2012-04-12
dc.date.published-print2012-07-01


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This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.
Except where otherwise noted, this item's license is described as This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at http://creativecommons.org/licenses/by-nc/3.0/.