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dc.contributor.authorIlg, Andrea
dc.contributor.authorBruno, Mark
dc.contributor.authorBeyer, Peter
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2014-11-11T14:32:44Z
dc.date.available2014-11-11T14:32:44Z
dc.date.issued2014-06-25
dc.identifier.citationIlg A, Bruno M, Beyer P, Al-Babili S (2014) Tomato carotenoid cleavage dioxygenases 1A and 1B: Relaxed double bond specificity leads to a plenitude of dialdehydes, mono-apocarotenoids and isoprenoid volatiles. FEBS Open Bio 4: 584-593. doi:10.1016/j.fob.2014.06.005.
dc.identifier.issn22115463
dc.identifier.pmid25057464
dc.identifier.doi10.1016/j.fob.2014.06.005
dc.identifier.urihttp://hdl.handle.net/10754/334629
dc.description.abstractThe biosynthetic processes leading to many of the isoprenoid volatiles released by tomato fruits are still unknown, though previous reports suggested a clear correlation with the carotenoids contained within the fruit. In this study, we investigated the activity of the tomato (Solanum lycopersicum) carotenoid cleavage dioxygenase (SlCCD1B), which is highly expressed in fruits, and of its homolog SlCCD1A. Using in vitro assays performed with purified recombinant enzymes and by analyzing products formed by the two enzymes in carotene-accumulating Escherichia coli strains, we demonstrate that SlCCD1A and, to a larger extent, SlCCD1B, have a very relaxed specificity for both substrate and cleavage site, mediating the oxidative cleavage of cis- and all-. trans-carotenoids as well as of different apocarotenoids at many more double bonds than previously reported. This activity gives rise to a plenitude of volatiles, mono-apocarotenoids and dialdehyde products, including cis-pseudoionone, neral, geranial, and farnesylacetone. Our results provide a direct evidence for a carotenoid origin of these compounds and point to CCD1s as the enzymes catalyzing the formation of the vast majority of tomato isoprenoid volatiles, many of which are aroma constituents. © 2014 The Authors.
dc.language.isoen
dc.publisherWiley
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/
dc.titleTomato carotenoid cleavage dioxygenases 1A and 1B: Relaxed double bond specificity leads to a plenitude of dialdehydes, mono-apocarotenoids and isoprenoid volatiles
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentPlant Science
dc.contributor.departmentPlant Science Program
dc.identifier.journalFEBS Open Bio
dc.identifier.pmcidPMC4096678
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionAlbert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germany
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personAl-Babili, Salim
refterms.dateFOA2018-06-13T16:04:48Z
dc.date.published-online2014-06-25
dc.date.published-print2014-01-01


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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
Except where otherwise noted, this item's license is described as This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).