Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID
Barbosa, Inês CR
Willige, Björn C
Port, Sarah A
de la Fuente van Bentem, Sergio
Schulze, Waltraud X
Hammes, Ulrich Z
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Plant Science Program
Permanent link to this recordhttp://hdl.handle.net/10754/334618
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AbstractThe development and morphology of vascular plants is critically determined by synthesis and proper distribution of the phytohormone auxin. The directed cell-to-cell distribution of auxin is achieved through a system of auxin influx and efflux transporters. PIN-FORMED (PIN) proteins are proposed auxin efflux transporters, and auxin fluxes can seemingly be predicted based on the-in many cells-asymmetric plasma membrane distribution of PINs. Here, we show in a heterologous Xenopus oocyte system as well as in Arabidopsis thaliana inflorescence stems that PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family. At the same time, we reveal that D6PKs and PID have differential phosphosite preferences. Our study suggests that PIN activation by protein kinases is a crucial component of auxin transport control that must be taken into account to understand auxin distribution within the plant.
CitationZourelidou M, Absmanner B, Weller B, Barbosa IC, Willige BC, et al. (2014) Auxin efflux by PIN-FORMED proteins is activated by two different protein kinases, D6 PROTEIN KINASE and PINOID. eLife 3. doi:10.7554/eLife.02860.
PublishereLife Sciences Publications, Ltd
PubMed Central IDPMC4091124
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- Dynamic PIN-FORMED auxin efflux carrier phosphorylation at the plasma membrane controls auxin efflux-dependent growth.
- Authors: Weller B, Zourelidou M, Frank L, Barbosa IC, Fastner A, Richter S, Jürgens G, Hammes UZ, Schwechheimer C
- Issue date: 2017 Jan 31
- Dynamic control of auxin transport-dependent growth by AGCVIII protein kinases.
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- Issue date: 2014 Dec
- D6PK AGCVIII kinases are required for auxin transport and phototropic hypocotyl bending in Arabidopsis.
- Authors: Willige BC, Ahlers S, Zourelidou M, Barbosa IC, Demarsy E, Trevisan M, Davis PA, Roelfsema MR, Hangarter R, Fankhauser C, Schwechheimer C
- Issue date: 2013 May
- D6 PROTEIN KINASE activates auxin transport-dependent growth and PIN-FORMED phosphorylation at the plasma membrane.
- Authors: Barbosa IC, Zourelidou M, Willige BC, Weller B, Schwechheimer C
- Issue date: 2014 Jun 23
- Phospholipid composition and a polybasic motif determine D6 PROTEIN KINASE polar association with the plasma membrane and tropic responses.
- Authors: Barbosa IC, Shikata H, Zourelidou M, Heilmann M, Heilmann I, Schwechheimer C
- Issue date: 2016 Dec 15