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dc.contributor.authorZhang, Gen
dc.contributor.authorHe, Li-Sheng
dc.contributor.authorWong, Yue Him
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-11-11T14:31:13Z
dc.date.available2014-11-11T14:31:13Z
dc.date.issued2013-07-29
dc.identifier.citationZhang G, He L-S, Wong YH, Qian P-Y (2013) MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK. PLoS ONE 8: e69510. doi:10.1371/journal.pone.0069510.
dc.identifier.issn1932-6203
dc.identifier.pmid23922727
dc.identifier.doi10.1371/journal.pone.0069510
dc.identifier.urihttp://hdl.handle.net/10754/334592
dc.description.abstractThe p38 mitogen-activated protein kinase (p38MAPK) plays a key role in larval settlement of the barnacle Amphibalanus amphitrite. To study the signaling pathway associated with p38MAPK during larval settlement, we sought to identify the upstream kinase of p38MAPK. Three MKKs (MKK3, MKK4 and MKK7) and three MAPKs (p38MAPK, ERK and JNK) in A. amphitrite were cloned and recombinantly expressed in E. coli. Through kinase assays, we found that MKK3, but not MKK4 or MKK7, phosphorylated p38MAPK. Furthermore, MKK3 activity was specific to p38MAPK, as it did not phosphorylate ERK or JNK. To further investigate the functional relationship between MKK3 and p38MAPK in vivo, we studied the localization of phospho-MKK3 (pMKK3) and MKK3 by immunostaining. Consistent with the patterns of p38MAPK and phospho-p38MAPK (pp38MAPK), pMKK3 and MKK3 mainly localized to the antennules of the cyprids. Western blot analysis revealed that pMKK3 levels, like pp38MAPK levels, were elevated at cyprid stage, compared to nauplii and juvenile stages. Moreover, pMKK3 levels increased after treatment with adult barnacle crude extracts, suggesting that MKK3 might mediate the stimulatory effects of adult barnacle extracts on the p38MAPK pathway. © 2013 Zhang et al.
dc.description.sponsorshipThis work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association (http://www.comra.org), an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016; http://www.kaust.edu.sa) and grants (GRF661611, GRF662413 and AoE/P-04/04-II) from the Research Grants Council (http://www.ugc.edu.hk/eng/rgc/index.htm) of the Hong Kong Special Administrative Region. The funders did not contribute to the experimental design, operation or manuscript writing. Additionally, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectmitogen activated protein kinase kinase 3
dc.subjectmitogen activated protein kinase kinase 4
dc.subjectmitogen activated protein kinase kinase 7
dc.subjectmitogen activated protein kinase p38
dc.subjectstress activated protein kinase
dc.subjectAmphibalanus amphitrite
dc.subjectbarnacle
dc.subjectcypris (larva)
dc.subjectenzyme activity
dc.subjectimmunohistochemistry
dc.subjectin vivo study
dc.subjectlarval development
dc.subjectnauplius (larva)
dc.subjectnucleotide sequence
dc.subjectprotein localization
dc.subjectprotein phosphorylation
dc.subjectWestern blotting
dc.subjectExtracellular Signal-Regulated MAP Kinases
dc.subjectJNK Mitogen-Activated Protein Kinases
dc.subjectLarva
dc.subjectMAP Kinase Kinase 3
dc.subjectMAP Kinase Kinase 4
dc.subjectMAP Kinase Kinase 7
dc.subjectp38 Mitogen-Activated Protein Kinases
dc.subjectThoracica
dc.titleMKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK
dc.typeArticle
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC3726695
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionKAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personZhang, Gen
kaust.personHe, Li-Sheng
kaust.personWong, Yue Him
kaust.personQian, Pei-Yuan
kaust.grant.numberSA-C0040
kaust.grant.numberUK-C0016
kaust.grant.programKAUST Global Collaborative Research Program
refterms.dateFOA2018-06-14T04:39:51Z


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