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    Characterization of Two 20kDa-Cement Protein (cp20k) Homologues in Amphibalanus amphitrite

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    Type
    Article
    Authors
    He, Li-Sheng
    Zhang, Gen
    Qian, Pei-Yuan cc
    KAUST Grant Number
    SA-C0040
    UK-C0016
    Date
    2013-05-22
    Permanent link to this record
    http://hdl.handle.net/10754/334590
    
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    Abstract
    The barnacle, Amphibalanus amphitrite, is a common marine fouling organism. Understanding the mechanism of barnacle adhesion will be helpful in resolving the fouling problem. Barnacle cement is thought to play a key role in barnacle attachment. Although several adult barnacle cement proteins have been identified in Megabalanus rosa, little is known about their function in barnacle settlement. In this study, two homologous 20k-cement proteins (cp20k) in Amphibalanus amphitrite, named Bamcp20k-1 and Bamcp20k-2, were characterized. The two homologues share primary sequence structure with proteins from other species including Megabalanus rosa and Fistulobalanus albicostatus. The conserved structure included repeated Cys domains and abundant charged amino acids, such as histidine. In this study we demonstrated that Bamcp20k-1 localized at the α secretory cells in the cyprid cement gland, while Bamcp20k-2 localized to the β secretory cells. The differential localizations suggest differential regulation for secretion from the secretory cells. Both Bamcp20k-1 and Bamcp20k-2 from cyprids dissolved in PBS. However, adult Bamcp20k-2, which was dominant in the basal shell of adult barnacles, was largely insoluble in PBS. Solubility increased in the presence of the reducing reagent Dithiothreitol (DTT), suggesting that the formation of disulfide bonds plays a role in Bamcp20k-2 function. In comparison, Bamcp20k-1, which was enriched in soft tissue, could not be easily detected in the shell and base by Western blot and easily dissolved in PBS. These differential solubilities and localizations indicate that Bamcp20k-1 and Bamcp20k-2 have distinct functions in barnacle cementing. © 2013 He et al.
    Citation
    He L-S, Zhang G, Qian P-Y (2013) Characterization of Two 20kDa-Cement Protein (cp20k) Homologues in Amphibalanus amphitrite. PLoS ONE 8: e64130. doi:10.1371/journal.pone.0064130.
    Sponsors
    The authors’ research grant from China Ocean Mineral Resources Research and Development Association (DY125-15-T-02), and King Abdullah University of Science and Technology (SA-C0040/UK-C0016) to P.Y. Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
    Publisher
    Public Library of Science (PLoS)
    Journal
    PLoS ONE
    DOI
    10.1371/journal.pone.0064130
    PubMed ID
    23717550
    PubMed Central ID
    PMC3661472
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0064130
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