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dc.contributor.authorChen, Zhang-Fan
dc.contributor.authorWang, Hao
dc.contributor.authorMatsumura, Kiyotaka
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-11-11T14:30:58Z
dc.date.available2014-11-11T14:30:58Z
dc.date.issued2012-02-13
dc.identifier.citationChen Z-F, Wang H, Matsumura K, Qian P-Y (2012) Expression of Calmodulin and Myosin Light Chain Kinase during Larval Settlement of the Barnacle Balanus amphitrite. PLoS ONE 7: e31337. doi:10.1371/journal.pone.0031337.
dc.identifier.issn1932-6203
dc.identifier.pmid22348072
dc.identifier.doi10.1371/journal.pone.0031337
dc.identifier.urihttp://hdl.handle.net/10754/334586
dc.description.abstractBarnacles are one of the most common organisms in intertidal areas. Their life cycle includes seven free-swimming larval stages and sessile juvenile and adult stages. The transition from the swimming to the sessile stages, referred to as larval settlement, is crucial for their survivor success and subsequent population distribution. In this study, we focused on the involvement of calmodulin (CaM) and its binding proteins in the larval settlement of the barnacle, Balanus (= Amphibalanus) amphitrite. The full length of CaM gene was cloned from stage II nauplii of B. amphitrite (referred to as Ba-CaM), encoding 149 amino acid residues that share a high similarity with published CaMs in other organisms. Quantitative real-time PCR showed that Ba-CaM was highly expressed in cyprids, the stage at which swimming larvae are competent to attach and undergo metamorphosis. In situ hybridization revealed that the expressed Ba-CaM gene was localized in compound eyes, posterior ganglion and cement glands, all of which may have essential functions during larval settlement. Larval settlement assays showed that both the CaM inhibitor compound 48/80 and the CaM-dependent myosin light chain kinase (MLCK) inhibitor ML-7 effectively blocked barnacle larval settlement, whereas Ca 2+/CaM-dependent kinase II (CaMKII) inhibitors did not show any clear effects. The subsequent real-time PCR assay showed a higher expression level of Ba-MLCK gene in larval stages than in adults, suggesting an important role of Ba-MLCK gene in larval development and competency. Overall, the results suggest that CaM and CaM-dependent MLCK function during larval settlement of B. amphitrite. © 2012 Chen et al.
dc.description.sponsorshipThis work was supported by an award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology and an Areas of Excellence (AoE) project of Hong Kong Special Administrative Region (AOE/P-04/04-2-II) to Pei-Yuan Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsChen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subject1 (5 iodo 1 naphthalenesulfonyl)hexahydro 1h 1,4 diazepine
dc.subjectamino acid
dc.subjectcalcium calmodulin dependent protein kinase II
dc.subjectcalmodulin
dc.subjectmyosin light chain kinase
dc.subjectprotein kinase inhibitor
dc.subjectcalmodulin
dc.subjectmyosin light chain kinase
dc.subjectBalanus amphitrite
dc.subjectbarnacle
dc.subjectCaM gene
dc.subjectcypris (larva)
dc.subjectenzyme activity
dc.subjectganglion
dc.subjectgene
dc.subjectgene expression profiling
dc.subjectgene location
dc.subjectin situ hybridization
dc.subjectlarval stage
dc.subjectmetamorphosis
dc.subjectmolecular cloning
dc.subjectnauplius (larva)
dc.subjectnucleotide sequence
dc.subjectprotein expression
dc.subjectquantitative analysis
dc.subjectreverse transcription polymerase chain reaction
dc.subjectbarnacle
dc.subjectbiosynthesis
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjectlarva
dc.subjectlife cycle stage
dc.subjectmetamorphosis
dc.subjectphysiology
dc.subjectBalanus amphitrite
dc.subjectCyprididae
dc.subjectThoracica
dc.subjectCalmodulin
dc.subjectCloning, Molecular
dc.subjectLarva
dc.subjectLife Cycle Stages
dc.subjectMetamorphosis, Biological
dc.subjectMyosin-Light-Chain Kinase
dc.subjectSequence Analysis, DNA
dc.subjectThoracica
dc.titleExpression of Calmodulin and Myosin Light Chain Kinase during Larval Settlement of the Barnacle Balanus amphitrite
dc.typeArticle
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC3278446
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionKAUST Global Collaborative Research Program, Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong SAR, China
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personWang, Hao
kaust.personChen, Zhangfan
kaust.personMatsumura, Kiyotaka
kaust.personQian, Pei-Yuan
kaust.grant.numberSA-C0040
kaust.grant.numberUK-C0016
kaust.grant.programKAUST Global Collaborative Research Program
refterms.dateFOA2018-06-14T04:39:26Z


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