Expression of Calmodulin and Myosin Light Chain Kinase during Larval Settlement of the Barnacle Balanus amphitrite
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AbstractBarnacles are one of the most common organisms in intertidal areas. Their life cycle includes seven free-swimming larval stages and sessile juvenile and adult stages. The transition from the swimming to the sessile stages, referred to as larval settlement, is crucial for their survivor success and subsequent population distribution. In this study, we focused on the involvement of calmodulin (CaM) and its binding proteins in the larval settlement of the barnacle, Balanus (= Amphibalanus) amphitrite. The full length of CaM gene was cloned from stage II nauplii of B. amphitrite (referred to as Ba-CaM), encoding 149 amino acid residues that share a high similarity with published CaMs in other organisms. Quantitative real-time PCR showed that Ba-CaM was highly expressed in cyprids, the stage at which swimming larvae are competent to attach and undergo metamorphosis. In situ hybridization revealed that the expressed Ba-CaM gene was localized in compound eyes, posterior ganglion and cement glands, all of which may have essential functions during larval settlement. Larval settlement assays showed that both the CaM inhibitor compound 48/80 and the CaM-dependent myosin light chain kinase (MLCK) inhibitor ML-7 effectively blocked barnacle larval settlement, whereas Ca 2+/CaM-dependent kinase II (CaMKII) inhibitors did not show any clear effects. The subsequent real-time PCR assay showed a higher expression level of Ba-MLCK gene in larval stages than in adults, suggesting an important role of Ba-MLCK gene in larval development and competency. Overall, the results suggest that CaM and CaM-dependent MLCK function during larval settlement of B. amphitrite. © 2012 Chen et al.
CitationChen Z-F, Wang H, Matsumura K, Qian P-Y (2012) Expression of Calmodulin and Myosin Light Chain Kinase during Larval Settlement of the Barnacle Balanus amphitrite. PLoS ONE 7: e31337. doi:10.1371/journal.pone.0031337.
SponsorsThis work was supported by an award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology and an Areas of Excellence (AoE) project of Hong Kong Special Administrative Region (AOE/P-04/04-2-II) to Pei-Yuan Qian. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PublisherPublic Library of Science (PLoS)
PubMed Central IDPMC3278446
CollectionsPublications Acknowledging KAUST Support
- Effects of calmodulin inhibitors on cyprid larvae of the barnacle, Balanus amphitrite.
- Authors: Yamamoto H, Tachibana A, Saikawa W, Nagano M, Matsumura K, Fusetani N
- Issue date: 1998 Jan 1
- Quantitative proteomics study of larval settlement in the Barnacle Balanus amphitrite.
- Authors: Chen ZF, Zhang H, Wang H, Matsumura K, Wong YH, Ravasi T, Qian PY
- Issue date: 2014
- Characterization and expression of calmodulin gene during larval settlement and metamorphosis of the polychaete Hydroides elegans.
- Authors: Chen ZF, Wang H, Qian PY
- Issue date: 2012 Aug
- The regulatory role of the NO/cGMP signal transduction cascade during larval attachment and metamorphosis of the barnacle Balanus (=Amphibalanus) amphitrite.
- Authors: Zhang Y, He LS, Zhang G, Xu Y, Lee OO, Matsumura K, Qian PY
- Issue date: 2012 Nov 1
- Transcriptomic analysis of neuropeptides and peptide hormones in the barnacle Balanus amphitrite: evidence of roles in larval settlement.
- Authors: Yan XC, Chen ZF, Sun J, Matsumura K, Wu RS, Qian PY
- Issue date: 2012
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