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dc.contributor.authorChandramouli, Kondethimmanahalli
dc.contributor.authorSoo, Lisa
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-11-11T14:30:47Z
dc.date.available2014-11-11T14:30:47Z
dc.date.issued2011-09-03
dc.identifier.citationChandramouli KH, Soo L, Qian P-Y (2011) Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I. Proteome Science 9: 51. doi:10.1186/1477-5956-9-51.
dc.identifier.issn1477-5956
dc.identifier.pmid21888661
dc.identifier.doi10.1186/1477-5956-9-51
dc.identifier.urihttp://hdl.handle.net/10754/334584
dc.description.abstractBackground: The spontaneous metamorphosis of the polychaete Capitella sp. I larvae into juveniles requires minor morphological changes, including segment formation, body elongation, and loss of cilia. In this study, we investigated changes in the expression patterns of both proteins and phosphoproteins during the transition from larvae to juveniles in this species. We used two-dimensional gel electrophoresis (2-DE) followed by multiplex fluorescent staining and MALDI-TOF mass spectrometry analysis to identify the differentially expressed proteins as well as the protein and phosphoprotein profiles of both competent larvae and juveniles.Results: Twenty-three differentially expressed proteins were identified in the two developmental stages. Expression patterns of two of those proteins were examined at the protein level by Western blot analysis while seven were further studied at the mRNA level by real-time PCR. Results showed that proteins related to cell division, cell migration, energy storage and oxidative stress were plentifully expressed in the competent larvae; in contrast, proteins involved in oxidative metabolism and transcriptional regulation were abundantly expressed in the juveniles.Conclusion: It is likely that these differentially expressed proteins are involved in regulating the larval metamorphosis process and can be used as protein markers for studying molecular mechanisms associated with larval metamorphosis in polychaetes. © 2011 Chandramouli et al; licensee BioMed Central Ltd.
dc.description.sponsorshipThe authors thank Mr. Y Zhang for his technical help in generating the 2-DE gels and Mr. Yue Him Wong for help with RT-PCR. We are also thankful to Dr. On On Lee for critically reviewing the manuscript and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and a grant from the Research Grants Council of the Hong Kong Special Administrative Region (AoE/P-04/04-II) to P.-Y. Qian.
dc.language.isoen
dc.publisherSpringer Nature
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.subject2-DEphos
dc.subjectCapitella sp. I
dc.subjectLarval metamorphosis
dc.subjectMultiplexed proteomics
dc.subjectPhoproteome
dc.subjectRT-PCR
dc.subjectactin
dc.subjectenolase phosphatase
dc.subjectheat shock protein 90
dc.subjectisocitrate dehydrogenase
dc.subjectisocitrate dehydrogenase isoenzyme
dc.subjectmessenger RNA
dc.subjectphosphatase
dc.subjectphosphoprotein
dc.subjectphosphoproteome
dc.subjectprotein
dc.subjectproteome
dc.subjectproton transporting adenosine triphosphate synthase
dc.subjectthioredoxin peroxidase
dc.subjecttropomyosin
dc.subjecttubulin
dc.subjecttyrosine 3 monooxygenase
dc.subjectunclassified drug
dc.subjectvitellogenin
dc.subjectaerobic metabolism
dc.subjectCapitella
dc.subjectcell division
dc.subjectcell migration
dc.subjectdown regulation
dc.subjectgel electrophoresis
dc.subjectgene expression
dc.subjectlarval stage
dc.subjectmatrix assisted laser desorption ionization time of flight mass spectrometry
dc.subjectmetamorphosis
dc.subjectoxidative stress
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectprotein metabolism
dc.subjectprotein phosphorylation
dc.subjectproteomics
dc.subjectreal time polymerase chain reaction
dc.subjecttranscription regulation
dc.subjectupregulation
dc.subjectWestern blotting
dc.subjectworm
dc.subjectCapitella
dc.subjectPolychaeta
dc.titleDifferential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.identifier.journalProteome Science
dc.identifier.pmcidPMC3180302
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionKAUST Global Collaborative Research, Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, China
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personChandramouli, Kondethimmanahalli
kaust.personSoo, Lisa
kaust.personQian, Pei-Yuan
kaust.grant.numberSA-C0040
kaust.grant.numberUK-C0016
kaust.grant.programKAUST Global Collaborative Research Program
refterms.dateFOA2018-06-14T04:39:25Z
dc.date.published-online2011-09-03
dc.date.published-print2011


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