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dc.contributor.authorChandramouli, Kondethimmanahalli
dc.contributor.authorMok, Flora SY
dc.contributor.authorWang, Hao
dc.contributor.authorQian, Pei-Yuan
dc.date.accessioned2014-11-11T14:30:44Z
dc.date.available2014-11-11T14:30:44Z
dc.date.issued2011-05-25
dc.identifier.citationChandramouli KH, Mok FS, Wang H, Qian P-Y (2011) Phosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa. BMC Developmental Biology 11: 31. doi:10.1186/1471-213X-11-31.
dc.identifier.issn1471-213X
dc.identifier.pmid21612608
dc.identifier.doi10.1186/1471-213X-11-31
dc.identifier.urihttp://hdl.handle.net/10754/334583
dc.description.abstractBackground: The metamorphosis of the spionid polychaete Pseudopolydora vexillosa includes spontaneous settlement onto soft-bottom habitats and morphogenesis that can be completed in a very short time. A previous study on the total changes to the proteome during the various developmental stages of P. vexillosa suggested that little or no de novo protein synthesis occurs during metamorphosis. In this study, we used multicolor fluorescence detection of proteins in 2-D gels for differential analysis of proteins and phosphoproteins to reveal the dynamics of post-translational modification proteins in this species. A combination of affinity chromatography, 2D-PAGE, and mass spectrometry was used to identify the phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles. Results: We reproducibly detected 210, 492, and 172 phosphoproteins in pre-competent larvae, competent larvae, and newly metamorphosed juveniles, respectively. The highest percentage of phosphorylation was observed during the competent larval stage. About 64 stage-specific phosphoprotein spots were detected in the competent stage, and 32 phosphoproteins were found to be significantly differentially expressed in the three stages. We identified 38 phosphoproteins, 10 of which were differentially expressed during metamorphosis. These phosphoproteins belonged to six categories of biological processes: (1) development, (2) cell differentiation and integrity, (3) transcription and translation, (4) metabolism, (5) protein-protein interaction and proteolysis, and (6) receptors and enzymes. Conclusion: This is the first study to report changes in phosphoprotein expression patterns during the metamorphosis of the marine polychaete P. vexillosa. The higher degree of phosphorylation during the process of attaining competence to settle and metamorphose may be due to fast morphological transitions regulated by various mechanisms. Our data are consistent with previous studies showing a high percentage of phosphorylation during competency in the barnacle Balanus amphitrite and the bryozoan Bugula neritina. The identified phosphoproteins may play an important role during metamorphosis, and further studies on the location and functions of important proteins during metamorphosis are warranted. © 2011 Chandramouli et al; licensee BioMed Central Ltd.
dc.description.sponsorshipThe authors thank Mr. Y. Zhang for his technical help in generating the 2-DE gels, and Dr. S. Arellano for critically reviewing the manuscript and Cherry Kwan for proof-reading the manuscript. This study was supported by an award from King Abdullah University of Science and Technology (SA-C0040/UK-C0016), a grant from the China Ocean Mineral Resource Research and Development Association (COMARRD06/07.Sc02), and grants from the Research Grants Council of the Hong Kong Special Administrative Region (N-HKUST602/09, 662408 and AoE/P-04/04-II) to P.-Y. Qian.
dc.language.isoen
dc.publisherSpringer Nature
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/
dc.subjectBalanus amphitrite
dc.subjectBryozoa
dc.subjectBugula neritina
dc.subjectPolychaeta
dc.subjectPseudopolydora
dc.subjectSpionida
dc.subjectThoracica
dc.subjectphosphoprotein
dc.subjectproteome
dc.subjectaffinity chromatography
dc.subjectchemistry
dc.subjectgrowth, development and aging
dc.subjecthistology
dc.subjectlarva
dc.subjectmass spectrometry
dc.subjectmetamorphosis
dc.subjectmethodology
dc.subjectphysiology
dc.subjectPolychaeta
dc.subjectprotein processing
dc.subjecttwo dimensional gel electrophoresis
dc.subjectChromatography, Affinity
dc.subjectElectrophoresis, Gel, Two-Dimensional
dc.subjectLarva
dc.subjectMass Spectrometry
dc.subjectMetamorphosis, Biological
dc.subjectPhosphoproteins
dc.subjectPolychaeta
dc.subjectProtein Processing, Post-Translational
dc.subjectProteome
dc.titlePhosphoproteome analysis during larval development and metamorphosis in the spionid polychaete Pseudopolydora vexillosa
dc.typeArticle
dc.identifier.journalBMC Developmental Biology
dc.identifier.pmcidPMC3115903
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionKAUST Global Collaborative Research Program, Division of Life Science, Hong Kong University of Science and Technology, Hong Kong SAR, China
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personWang, Hao
kaust.personChandramouli, Kondethimmanahalli
kaust.personMok, FloraSy
kaust.personQian, Pei-Yuan
kaust.grant.numberSA-C0040
kaust.grant.numberUK-C0016
kaust.grant.programKAUST Global Collaborative Research Program
refterms.dateFOA2018-06-14T04:39:20Z


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