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dc.contributor.authorKremb, Stephan
dc.contributor.authorHelfer, Markus
dc.contributor.authorKraus, Birgit
dc.contributor.authorWolff, Horst
dc.contributor.authorWild, Christian
dc.contributor.authorSchneider, Martha
dc.contributor.authorVoolstra, Christian R.
dc.contributor.authorBrack-Werner, Ruth
dc.date.accessioned2014-11-11T14:30:32Z
dc.date.available2014-11-11T14:30:32Z
dc.date.issued2014-08-21
dc.identifier.citationKremb S, Helfer M, Kraus B, Wolff H, Wild C, et al. (2014) Aqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells. PLoS ONE 9: e103895. doi:10.1371/journal.pone.0103895.
dc.identifier.issn1932-6203
dc.identifier.pmid25144758
dc.identifier.doi10.1371/journal.pone.0103895
dc.identifier.urihttp://hdl.handle.net/10754/334578
dc.description.abstractIn recent years, marine algae have emerged as a rich and promising source of molecules with potent activities against various human pathogens. The widely distributed brown alga Lobophora variegata that is often associated with tropical coral reefs exerts strong antibacterial and antiprotozoal effects, but so far has not been associated with specific anti-viral activities. This study investigated potential HIV-1 inhibitory activity of L. variegata collected from different geographical regions, using a cell-based full replication HIV-1 reporter assay. Aqueous L. variegata extracts showed strong inhibitory effects on several HIV-1 strains, including drug-resistant and primary HIV-1 isolates, and protected even primary cells (PBMC) from HIV-1-infection. Anti-viral potency was related to ecological factors and showed clear differences depending on light exposition or epiphyte growth. Assays addressing early events of the HIV-1 replication cycle indicated that L. variegata extracts inhibited entry of HIV-1 into cells at a pre-fusion step possibly by impeding mobility of virus particles. Further characterization of the aqueous extract demonstrated that even high doses had only moderate effects on viability of cultured and primary cells (PBMCs). Imaging-based techniques revealed extract effects on the plasma membrane and actin filaments as well as induction of apoptosis at concentrations exceeding EC50 of anti-HIV-1 activity by more than 400 fold. In summary, we show for the first time that L. variegata extracts inhibit HIV-1 entry, thereby suggesting this alga as promising source for the development of novel HIV-1 inhibitors.
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rightsArchived with thanks to PLoS ONE
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleAqueous Extracts of the Marine Brown Alga Lobophora variegata Inhibit HIV-1 Infection at the Level of Virus Entry into Cells
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.contributor.departmentReef Genomics Lab
dc.identifier.journalPLoS ONE
dc.identifier.pmcidPMC4140698
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionInstitute of Virology, Helmholtz Zentrum Muenchen, Neuherberg, Germany
dc.contributor.institutionInstitute of Pharmacy, University of Regensburg, Regensburg, Germany
dc.contributor.institutionCarl Zeiss Microscopy GmbH, Goettingen, Germany
dc.contributor.institutionCoral Reef Ecology Group (CORE), Leibniz Center for Tropical Marine Ecology (ZMT), Bremen, Germany
dc.contributor.institutionUniversity of Bremen, Faculty of Biology and Chemistry, Bremen, Germany
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)
kaust.personVoolstra, Christian R.
refterms.dateFOA2018-06-14T04:39:09Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.